Rabbit anti-Human, Mouse AGER Polyclonal Antibody | anti-AGER antibody

AGER, NT (AGER, RAGE, Advanced glycosylation end product-specific receptor, Receptor for advanced glycosylation end products) (APC)

Cat. Num. AAA6272191

• Gene Names: AGER; RAGE
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: AGER; Polyclonal Antibody; NT (AGER; RAGE; Advanced glycosylation end product-specific receptor; Receptor for advanced glycosylation end products) (APC); Receptor for advanced glycosylation end products; anti-AGER antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human AGER. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-AGER antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 24-52 in the N-terminal region of human AGER. Cellular Localization: Cell membrane; Single-pass type I membrane protein
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of mouse lung tissue lysates (35ug/lane) usingMBS645609. AGER (arrow) was detected usingMBS645609.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma using MBS645609 followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Flow Cytometry (FC/FACS)

(Flow Cytometry of U251 cells (bottom histogram) using MBS645609 compared to a negative control cell (top histogram). FITC-conjugated goat anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-AGER antibody

Antibodies; Receptors

References

1. Rojas, A., et al. Carcinogenesis 31(3):334-341(2010). 2. Engelen, L., et al. J. Hypertens. 28(2):285-293(2010). 3. Poon, P.Y., et al. Clin. Nephrol. 73(1):44-50(2010). 4. Hancock, D.B., et al. Nat. Genet. 42(1):45-52(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 332800988
• NCBI GeneID: 177
• NCBI Accession #: NP_001193895.1
• NCBI GenBank Nucleotide #: NM_001206966.1
• UniProt Accession #: Q15109; Q15279; Q3L1R7; Q86SN1; Q9H2X7; Q9Y3R3; A2BFI7; A6NKF0; B0V176
• Molecular Weight: 42,803 Da
• NCBI Official Full Name: advanced glycosylation end product-specific receptor isoform 6
• NCBI Official Synonym Full Names: advanced glycosylation end product-specific receptor
• NCBI Official Symbol: AGER
• NCBI Official Synonym Symbols: RAGE
• NCBI Protein Information: advanced glycosylation end product-specific receptor; RAGE isoform sRAGE-delta; RAGE isoform NtRAGE-delta
• UniProt Protein Name: Advanced glycosylation end product-specific receptor
• Protein Family: Advanced glycosylation end product-specific receptor
• UniProt Gene Name: AGER
• UniProt Synonym Gene Names: RAGE
• UniProt Entry Name: RAGE_HUMAN

NCBI Description

The advanced glycosylation end product (AGE) receptor encoded by this gene is a member of the immunoglobulin superfamily of cell surface receptors. It is a multiligand receptor, and besides AGE, interacts with other molecules implicated in homeostasis, development, and inflammation, and certain diseases, such as diabetes and Alzheimer's disease. Many alternatively spliced transcript variants encoding different isoforms, as well as non-protein-coding variants, have been described for this gene (PMID:18089847). [provided by RefSeq, May 2011]

Uniprot Description

RAGE: Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF- alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. Interacts with S100B, S100A1 and APP. Interacts with S100A12. Endothelial cells. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Motility/polarity/chemotaxis; Cell cycle regulation; Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: integral to plasma membrane; extracellular region; plasma membrane

Molecular Function: identical protein binding; protein binding; transmembrane receptor activity; receptor activity

Biological Process: cell surface receptor linked signal transduction; response to wounding; innate immune response; inflammatory response; neurite development; induction of positive chemotaxis; activation of NF-kappaB transcription factor