Rabbit anti-Human AGBL5 Polyclonal Antibody | anti-AGBL5 antibody

AGBL5, NT (AGBL5, CCP5, Cytosolic carboxypeptidase-like protein 5, ATP/GTP-binding protein-like 5) (APC)

Cat. Num. AAA6272180

• Gene Names: AGBL5; CCP5
• Reactivity: Human
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: AGBL5; Polyclonal Antibody; NT (AGBL5; CCP5; Cytosolic carboxypeptidase-like protein 5; ATP/GTP-binding protein-like 5) (APC); ATP/GTP-binding protein-like 5; anti-AGBL5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-AGBL5 antibody: Immunofluorescence (IF), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IF: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: AGBL5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 106-135 amino acids from the N-terminal region of human AGBL5.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(AGBL5 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the AGBL5 antibody detected the AGBL5 protein (arrow).)

Immunohistochemistry (IHC)

(AGBL5 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of AGBL5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of AGBL5 Antibody (N-term) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-AGBL5 antibody

AGBL5 has a function in the processing of cytosolic proteins such as alpha tubulin, which is known to be modified by the removal of a C terminal tyrosine. It is expressed in the brain. There are three named isoforms.

Product Categories/Family for anti-AGBL5 antibody

Antibodies; Enzymes; Carboxypeptidase

References

Bailey, S.D., et al. Diabetes Care (2010). In press: Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Wang, A.G., et al. Biochem. Biophys. Res. Commun. 345(3):1022-1032(2006). Simpson, J.C., et al. EMBO Rep. 1(3):287-292(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 118421091
• NCBI GeneID: 60509
• NCBI Accession #: NP_068603.4
• NCBI GenBank Nucleotide #: NM_021831.5
• UniProt Accession #: Q8NDL9; Q53SW0; Q53SZ0; Q96IK8; Q9H6V0; Q9H8P8; A2VDI7; B7WPG9; B7Z7I7; D6W548
• Molecular Weight: 80,016 Da
• NCBI Official Full Name: cytosolic carboxypeptidase-like protein 5 isoform 1
• NCBI Official Synonym Full Names: ATP/GTP binding protein-like 5
• NCBI Official Symbol: AGBL5
• NCBI Official Synonym Symbols: CCP5
• NCBI Protein Information: cytosolic carboxypeptidase-like protein 5; cytosolic carboxypeptidase 5; ATP/GTP-binding protein-like 5
• UniProt Protein Name: Cytosolic carboxypeptidase-like protein 5
• Protein Family: Cytosolic carboxypeptidase-like protein
• UniProt Gene Name: AGBL5
• UniProt Synonym Gene Names: CCP5
• UniProt Entry Name: CBPC5_HUMAN

Uniprot Description

Function: Metallocarboxypeptidase that mediates tubulin deglutamylation. Specifically catalyzes the deglutamylation of the branching point glutamate side chains generated by post-translational glutamylation in proteins such as tubulins. In contrast, it is not able to act as a long-chain deglutamylase that shortens long polyglutamate chains, a process catalyzed by AGTPBP1/ CCP1, AGBL1/CCP4 and AGBL4/CCP6

By similarity.

Cofactor: Binds 1 zinc ion per subunit

By similarity.

Subcellular location: Cytoplasm › cytosol

By similarity. Nucleus

By similarity. Note: Mainly cytoplasmic. Slight accumulation in the nucleus is observed

By similarity.

Tissue specificity: Expressed in brain.

Sequence similarities: Belongs to the peptidase M14 family.

Sequence caution: The sequence AAX93164.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAY14655.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAB15151.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.