Immunofluorescence (IF)
(Immunofluorescence staining of AFF3 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human AFF3 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.)
Rabbit anti-Human AFF3 Polyclonal Antibody | anti-AFF3 antibody
Anti-AFF3 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence
Purity
Protein A & Antigen Affinity
Synonyms
AFF3; Polyclonal Antibody; Anti-AFF3 Antibody; Rabbit Polyclonal; AFF3 Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-LAF4 Antibody; Anti-MLLT2-like Antibody; anti-AFF3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human AFF3
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-AFF3 antibody
Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
ICC/IF: 1:100-1:500
Immunogen
E Coli-derived Human AFF3 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human AFF3 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Immunofluorescence (IF)
(Immunofluorescence staining of AFF3 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human AFF3 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.)
Immunofluorescence (IF)
(Immunofluorescence staining of AFF3 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human AFF3 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.)
Related Product Information for anti-AFF3 antibody
AFF3 (AF4/FMR2 family member 3) is a gene on chromosome 2q11.2-q12 that encodes a tissue-restricted nuclear transcriptional activator preferentially expressed in lymphoid tissue, which may function in lymphoid development and oncogenesis. AFF3 is enriched at both the intergenic-differentially methylated region (IG-DMR) and the Meg3 enhancer within the imprinted Dlk1-Dio3 locus to regulate the allele-specific gene expression in this locus. AFF3 is enriched at gamete differentially methylated regions (DMRs) of the imprinted loci and regulates the imprinted gene expression. AFF3 is a new target of Wnt/beta-catenin pathway involved in adrenocortical cancer (ACC), acting on transcription and RNA splicing.
References
Wang Y, et al. (2017) A permissive chromatin state regulated by zfp281-aff3 in controlling the imprinted meg3 polycistron. Nucleic Acids Res 45 (3): 1177-1185.
Shi Y, et al. (2018) Aff3 upregulation mediates tamoxifen resistance in breast cancers. J Exp Clin Cancer Res 37 (1): 254.
Lefevre L, et al. (2015) Combined transcriptome studies identify aff3 as a mediator of the oncogenic effects of beta-catenin in adrenocortical carcinoma. Oncogenesis 4 (7): e161.
Shi Y, et al. (2018) Aff3 upregulation mediates tamoxifen resistance in breast cancers. J Exp Clin Cancer Res 37 (1): 254.
Lefevre L, et al. (2015) Combined transcriptome studies identify aff3 as a mediator of the oncogenic effects of beta-catenin in adrenocortical carcinoma. Oncogenesis 4 (7): e161.
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Product Notes
The AFF3 (Catalog #AAA8110016) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-AFF3 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AFF3 can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunofluorescence (IF). ICC/IF: 1:100-1:500. Researchers should empirically determine the suitability of the AFF3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AFF3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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