Rabbit anti-Human, Mouse AF9 Polyclonal Antibody | anti-MLLT3 antibody
AF9 Antibody
Gene Names
MLLT3; AF9; YEATS3
Reactivity
Human, Mouse
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
AF9; Polyclonal Antibody; AF9 Antibody; AF 9; AF9_HUMAN; ALL1 fused gene from chromosome 9 protein; ALL1-fused gene from chromosome 9 protein; FLJ2035; MLLT3; Myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein; Protein AF-9; YEATS 3; YEATS domain containing protein 3; YEATS domain-containing protein 3; YEATS3; anti-MLLT3 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
AF9 Antibody detects endogenous levels of total AF9.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-MLLT3 antibody
ELISA (EIA)(peptide)
Application Notes
ELISA(peptide): 1:20000-1:40000
Immunogen
A synthesized peptide derived from Human MYOCD.
Conjugation
Unconjugated
Similarity
The YEATS domain specifically recognizes and binds acylated histones, with a marked preference histones that are crotonylated (PubMed:27105114, PubMed:27545619). Also binds histone H3 acetylated at 'Lys-9' (H3K9ac), but with lower affinity (PubMed:25417107, PubMed:27105114).
Subunit Structure
Component of the super elongation complex (SEC), at least composed of EAF1, EAF2, CDK9, MLLT3/AF9, AFF (AFF1 or AFF4), the P-TEFb complex and ELL (ELL, ELL2 or ELL3). Interacts with BCOR. Interacts with CBX8. Interacts with ALKBH4.
Function
Chromatin reader component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. Specifically recognizes and binds acylated histone H3, with a marked preference for histone H3 that is crotonylated. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. Recognizes and binds histone H3 crotonylated at 'Lys-9' (H3K9cr), and with slightly lower affinity histone H3 crotonylated at 'Lys-18' (H3K18cr). Also recognizes and binds histone H3 acetylated at 'Lys-9' (H3K9ac), but with lower affinity than crotonylated histone H3. In the SEC complex, MLLT3 is required to recruit the complex to crotonylated histones.
Note
Colocalizes with acylated histone H3 (PubMed:25417107, PubMed:27105114). Colocalizes with histone H3 crotonylated at 'Lys-18' (H3K18cr) (PubMed:27105114).
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Product Categories/Family for anti-MLLT3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
568
NCBI Official Full Name
Protein AF-9
NCBI Official Synonym Full Names
myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 3
NCBI Official Symbol
MLLT3
NCBI Official Synonym Symbols
AF9; YEATS3
NCBI Protein Information
protein AF-9; YEATS domain-containing protein 3; ALL1-fused gene from chromosome 9 protein; myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog); translocated to, 3
UniProt Protein Name
Protein AF-9
Protein Family
UniProt Gene Name
MLLT3
UniProt Synonym Gene Names
AF9; YEATS3
UniProt Entry Name
AF9_HUMAN
Uniprot Description
MLLT3: A chromosomal aberration involving MLLT3 is associated with acute leukemias. Translocation t(9;11)(p22;q23) with MLL/HRX. The result is a rogue activator protein.
Protein type: Cell development/differentiation; Oncoprotein
Chromosomal Location of Human Ortholog: 9p22
Cellular Component: transcription elongation factor complex; nucleus
Molecular Function: protein binding
Biological Process: anterior/posterior pattern formation; segment specification; regulation of transcription, DNA-dependent; transcription, DNA-dependent
Research Articles on MLLT3
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Product Notes
The MLLT3 mllt3 (Catalog #AAA9629635) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AF9 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's AF9 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA)(peptide). ELISA(peptide): 1:20000-1:40000. Researchers should empirically determine the suitability of the MLLT3 mllt3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AF9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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