Rabbit anti-Human AF5q31 Polyclonal Antibody | anti-AF5q31 antibody

AF5q31, NT (AFF4, AF5Q31, MCEF, AF4/FMR2 family member 4, ALL1-fused gene from chromosome 5q31 protein, Major CDK9 elongation factor-associated protein) (APC)

Cat. Num. AAA6272037

• Gene Names: AFF4; MCEF; AF5Q31
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: AF5q31; Polyclonal Antibody; NT (AFF4; AF5Q31; MCEF; AF4/FMR2 family member 4; ALL1-fused gene from chromosome 5q31 protein; Major CDK9 elongation factor-associated protein) (APC); AFF4; Major CDK9 elongation factor-associated protein; anti-AF5q31 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-AF5q31 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: AF5q31 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human AF5q31.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of AF5q31 Antibody (N-term) in HL-60 cell line lysates (35ug/lane). AF5q31 (arrow) was detected using the purified Pab.)

Product Categories/Family for anti-AF5q31 antibody

Antibodies; CD Markers

References

Lin, C., et al. Mol. Cell 37(3):429-437(2010). Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007). Matsuoka, S., et al. Science 316(5828):1160-1166(2007). Niedzielski, M.F., et al. Int. J. Biol. Sci. 3(4):225-236(2007). Olsen, J.V., et al. Cell 127(3):635-648(2006). Olsen, J.V., et al. Cell 127(3):635-648(2006). Estable, M.C., et al. J. Biomed. Sci. 9(3):234-245(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 74720814
• NCBI GeneID: 27125
• UniProt Accession #: Q9UHB7; Q498B2; Q59FB3; Q6P592; Q8TDR1; Q9P0E4; B2RP19; B7WPD2
• Molecular Weight: 1163
• NCBI Official Full Name: AF4/FMR2 family member 4
• NCBI Official Synonym Full Names: AF4/FMR2 family, member 4
• NCBI Official Symbol: AFF4
• NCBI Official Synonym Symbols: MCEF; AF5Q31
• NCBI Protein Information: AF4/FMR2 family member 4; ALL1-fused gene from chromosome 5q31 protein; major CDK9 elongation factor-associated protein
• UniProt Protein Name: AF4/FMR2 family member 4
• UniProt Gene Name: AFF4
• UniProt Synonym Gene Names: AF5Q31; MCEF; Protein AF-5q31
• UniProt Entry Name: AFF4_HUMAN

NCBI Description

The protein encoded by this gene belongs to the AF4 family of transcription factors involved in leukemia. It is a component of the positive transcription elongation factor b (P-TEFb) complex. A chromosomal translocation involving this gene and MLL gene on chromosome 11 is found in infant acute lymphoblastic leukemia with ins(5;11)(q31;q31q23). [provided by RefSeq, Oct 2011]

Uniprot Description

MCEF: a putative transcription factor. Component of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor b (P-TEFb). Genetic fusion of its gene with MLL has been observed in a subset of acute lymphoblastic leukemia patients. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Oncoprotein; Transcription factor

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: nucleoplasm; transcription elongation factor complex; mitochondrion; nucleolus; nucleus

Molecular Function: protein binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; spermatid development

Disease: Chops Syndrome