Rabbit Advanced Glycosylation Endproduct Polyclonal Antibody
Advanced Glycosylation Endproduct (AGE, CML, Ne-Carboxymethyllysine)
Applications
ELISA
Purity
SerumSerum
Synonyms
Advanced Glycosylation Endproduct; Polyclonal Antibody; Advanced Glycosylation Endproduct (AGE; CML; Ne-Carboxymethyllysine); Anti -Advanced Glycosylation Endproduct (AGE; anti-Advanced Glycosylation Endproduct antibody
Host
Rabbit
Clonality
Polyclonal
Specificity
Recognizes Ne-Carboxymethyllysine (CML)
Purity/Purification
Serum
Serum
Serum
Form/Format
Supplied as a lyophilized powder in PBS, pH 7.2. Reconstitute in ddH2O.
Applicable Applications for anti-Advanced Glycosylation Endproduct antibody
ELISA (EL/EIA)
Application Notes
Suitable for use in ELISA.
Dilution: ELISA: 1:1000
Dilution: ELISA: 1:1000
Immunogen
Nepsilon -(Carboxymethyl)lysine KLH-conjugated
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-Advanced Glycosylation Endproduct antibody
Advanced glycation endproducts induce changes in glucose consumption, lactate production, and ATP levels in SH-SY5Y neuroblastoma cells by a redox-sensitive mechanism. Advanced glycation endproducts (AGEs) accumulate on long-lived proteins, including beta-amyloid plaques in Alzheimer's disease. They are suggested to contribute to neuronal dysfunction and cell death. A recent study at Neuroimmunological Cell Biology Unit, Leipzig, Germany investigated the effects of a model AGE upon glucose metabolism and energy production in a neuroblastoma cell line. AGEs decrease cellular ATP levels and increase glucose consumption and lactate production. All of the AGE-induced metabolic changes can be attenuated by antioxidants such as (R+)-alpha-lipoic acid and 17beta-estradiol. These antioxidants may become useful drugs against (AGE-mediated) effects in neurodegeneration through their positive effects on cellular energy metabolism. Reaction of amino groups in protein with glucose leads to the formation of advanced glycation end products (AGE) through the early products such as Schiff base and Amadori rearrangement products. Recent immunological studies using anti-AGE antibody demonstrated the presence of AGE-modified proteins in several human tissues:(i) human lens (non-diabetic and non-cataractous), (ii) renal proximal tubules in patients with diabetic nephropathy and chronic renal failure, (iii) diabetic retina, (iv) peripheral nerves of diabetic neuropathy, (v) atherosclerotic lesions of arterial walls, (vi) b2-microglobulin forming anyloid fibrils in patients with hemodialysis-related amyloidosis, (vii) senile plaques of patients with Alzheimer disease, (viii) peritoneum of CAPD patients, (ix) skin elastin in actinic elastosis, and (x) ceroid/lipofusion deposits. These results suggest a potential role of AGE-modification in normal aging as well as age-enhanced disease processes.
Product Categories/Family for anti-Advanced Glycosylation Endproduct antibody
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Product Notes
The Advanced Glycosylation Endproduct (Catalog #AAA619018) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Advanced Glycosylation Endproduct can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA). Suitable for use in ELISA. Dilution: ELISA: 1:1000. Researchers should empirically determine the suitability of the Advanced Glycosylation Endproduct for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Advanced Glycosylation Endproduct, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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