Human IMP3, U3 small nucleolar ribonucleoprotein, homolog (yeast) ELISA Kit | IMP3 elisa kit

Human U3 small nucleolar ribonucleoprotein protein IMP3, IMP3 ELISA Kit

Cat. Num. AAA934670

• Gene Names: IMP3; BRMS2; MRPS4; C15orf12
• Reactivity: Human
• Synonyms: IMP3; U3 small nucleolar ribonucleoprotein; homolog (yeast); Human U3 small nucleolar ribonucleoprotein protein IMP3; IMP3 ELISA Kit; Human U3 small nucleolar ribonucleoprotein protein IMP3 (IMP3) ELISA kit; BRMS2; C15orf12; DKFZp586L0118; FLJ10968; MRPS4; OTTHUMP00000183524; U3 snoRNP protein 3 homolog; IMP3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human IMP3. No significant cross-reactivity or interference between human IMP3 and analogues was observed.
• Sequence Length: 184

• Samples: Serum, plasma, tissue homogenates, Cell lysates
• Assay Type: Sandwich
• Detection Range: 39 pg/ml-2500 pg/ml.
• Sensitivity: The minimum detectable dose of human IMP3 is typically less than 9.75 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IMP3 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IMP3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IMP3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IMP3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IMP3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 8922794
• NCBI GeneID: 55272
• NCBI Accession #: NP_060755.1
• NCBI GenBank Nucleotide #: NM_018285.3
• UniProt Accession #: Q9NV31; Q53G10; B3KQ67
• Molecular Weight: 21,850 Da
• NCBI Official Full Name: U3 small nucleolar ribonucleoprotein protein IMP3
• NCBI Official Synonym Full Names: IMP3, U3 small nucleolar ribonucleoprotein
• NCBI Official Symbol: IMP3
• NCBI Official Synonym Symbols: BRMS2; MRPS4; C15orf12
• NCBI Protein Information: U3 small nucleolar ribonucleoprotein protein IMP3; U3 snoRNP protein IMP3; U3 snoRNP protein 3 homolog; IMP3, U3 small nucleolar ribonucleoprotein, homolog
• UniProt Protein Name: U3 small nucleolar ribonucleoprotein protein IMP3
• Protein Family: U3 small nucleolar ribonucleoprotein protein
• UniProt Gene Name: IMP3
• UniProt Synonym Gene Names: C15orf12; MRPS4; U3 snoRNP protein IMP3
• UniProt Entry Name: IMP3_HUMAN

NCBI Description

This gene encodes the human homolog of the yeast Imp3 protein. The protein localizes to the nucleoli and interacts with the U3 snoRNP complex. The protein contains an S4 domain. [provided by RefSeq, Jul 2008]

Uniprot Description

IMP3: Component of the 60-80S U3 small nucleolar ribonucleoprotein (U3 snoRNP). Required for the early cleavages during pre-18S ribosomal RNA processing. Belongs to the ribosomal protein S4P family.

Protein type: RNA-binding; Nucleolus; RNA processing

Chromosomal Location of Human Ortholog: 15q24

Cellular Component: small subunit processome; small ribosomal subunit; nucleolus; nucleus

Molecular Function: snoRNA binding; rRNA binding; protein binding; structural constituent of ribosome

Biological Process: positive regulation of translational fidelity; rRNA processing