Rabbit anti-Human ADRB2 Polyclonal Antibody | anti-ADRB2 antibody

ADRB2 (S364) (ADRB2R, ADRBR, B2AR, BAR, Beta-2 Adrenergic Receptor, Beta-2 Adrenoceptor, Beta-2 Adrenoreceptor, BETA2AR, Adrenergic, beta-2-, Receptor, Surface) (FITC)

Cat. Num. AAA6461800

• Gene Names: ADRB2; BAR; B2AR; ADRBR; ADRB2R; BETA2AR
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: ADRB2; Polyclonal Antibody; ADRB2 (S364) (ADRB2R; ADRBR; B2AR; BAR; Beta-2 Adrenergic Receptor; Beta-2 Adrenoceptor; Beta-2 Adrenoreceptor; BETA2AR; Adrenergic; beta-2-; Receptor; Surface) (FITC); anti-ADRB2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ADRB2.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-ADRB2 antibody: FLISA, Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 345-373 of human ADRB2.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(All lanes: MBS626831 (1:1000), lane 1: Hela whole cell lysate, lane 2: HepG2 whole cell lysate, lane 3: HUVEC whole cell lysate, lane 4: Human liver lysate, lane 5: NCI-H460 whole cell lysate, lane 6: PC-3 whole cell lysate, lane 7: A431 whole cell lysate. Lysates/proteins at 20ug/lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000. Predicted band size: 46kD. Blocking/Dilution buffer: 5% NFDM/TBST.)

Testing Data

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Testing Data

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Related Product Information for anti-ADRB2 antibody

ADRB2, beta-2-adrenergic receptor which is a member of the G protein-coupled receptor superfamily. This receptor is directly associated with one of its ultimate effectors, the class C L-type calcium channel Ca(V)1.2. This receptor-channel complex also contains a G protein, an adenylyl cyclase, cAMP-dependent kinase, and the counterbalancing phosphatase, PP2A. The assembly of the signaling complex provides a mechanism that ensures specific and rapid signaling by this G protein-coupled receptor. This protein is intronless.

Product Categories/Family for anti-ADRB2 antibody

Antibodies; Receptors

References

1. Kobilka B.K., Dixon R.A.F.Proc. Natl. Acad. Sci. USA 84:46-50(1987). 2. Emorine L.J., Marullo S.Proc. Natl. Acad. Sci. USA 84:6995-6999(1987) 3. Kobilka B.K., Frielle T.J. Biol. Chem. 262:7321-7327(1987).

NCBI and Uniprot Product Information

• NCBI GI #: 4501969
• NCBI GeneID: 154
• NCBI Accession #: NP_000015
• NCBI GenBank Nucleotide #: NM_000024
• UniProt Accession #: P07550; O14823; O14824; O14825; O14826; Q4JG18; Q53GA6; Q6GMT4; Q6P4D8; Q8NEQ9; B0LPE4; B2R7X2
• Molecular Weight: 46,459 Da
• NCBI Official Full Name: beta-2 adrenergic receptor
• NCBI Official Synonym Full Names: adrenoceptor beta 2, surface
• NCBI Official Symbol: ADRB2
• NCBI Official Synonym Symbols: BAR; B2AR; ADRBR; ADRB2R; BETA2AR
• NCBI Protein Information: beta-2 adrenergic receptor; adrenergic, beta-2-, receptor, surface; adrenoceptor beta 2 surface; beta-2 adrenoceptor; beta-2 adrenoreceptor; catecholamine receptor
• UniProt Protein Name: Beta-2 adrenergic receptor
• Protein Family: Beta-2 adrenergic receptor
• UniProt Gene Name: ADRB2
• UniProt Synonym Gene Names: ADRB2R; B2AR; Beta-2 adrenoceptor
• UniProt Entry Name: ADRB2_HUMAN

Uniprot Description

ADRB2: Beta-adrenergic receptors mediate the catecholamine- induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine. Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB2 sub-subfamily.

Protein type: Receptor, GPCR; GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 5q31-q32

Cellular Component: integral to plasma membrane; lysosome; apical plasma membrane; plasma membrane; nucleus; endosome; receptor complex

Molecular Function: protein binding; protein homodimerization activity; potassium channel regulator activity; beta2-adrenergic receptor activity; norepinephrine binding; adenylate cyclase binding

Biological Process: arrestin mediated desensitization of G-protein coupled receptor protein signaling pathway; receptor-mediated endocytosis; diet induced thermogenesis; negative regulation of multicellular organism growth; transmembrane receptor protein tyrosine kinase activation (dimerization); regulation of vasodilation; adenylate cyclase activation; regulation of sodium ion transport; positive regulation of bone mineralization; G-protein signaling, adenylate cyclase activating pathway; G-protein signaling, coupled to cAMP nucleotide second messenger; cell surface receptor linked signal transduction; positive regulation of MAPKKK cascade; positive regulation of protein ubiquitination; heat generation; negative regulation of smooth muscle contraction; brown fat cell differentiation; endosome to lysosome transport; positive regulation of transcription from RNA polymerase II promoter; response to cold; bone resorption; norepinephrine-epinephrine vasodilation involved in regulation of systemic arterial blood pressure

Disease: Obesity; Asthma, Susceptibility To