Goat ADRA2A Polyclonal Antibody | anti-ADRA2A antibody
Goat anti-ADRA2A Antibody
Western Blot: Preliminary experiments gave a band at approx 37kDa and a minor band at approx 23kDa in Human Brain lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 49. 0kDa according to NP_000672.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
Immunofluorescence: This product has been successfully used in IF on Mouse primary DRG cultured neurons.
NCBI and Uniprot Product Information
NCBI Description
Alpha-2-adrenergic receptors are members of the G protein-coupled receptor superfamily. They include 3 highly homologous subtypes: alpha2A, alpha2B, and alpha2C. These receptors have a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. Studies in mouse revealed that both the alpha2A and alpha2C subtypes were required for normal presynaptic control of transmitter release from sympathetic nerves in the heart and from central noradrenergic neurons; the alpha2A subtype inhibited transmitter release at high stimulation frequencies, whereas the alpha2C subtype modulated neurotransmission at lower levels of nerve activity. This gene encodes alpha2A subtype and it contains no introns in either its coding or untranslated sequences. [provided by RefSeq, Jul 2008]
Uniprot Description
ADRA2A: Alpha-2 adrenergic receptors mediate the catecholamine- induced inhibition of adenylate cyclase through the action of G proteins. The rank order of potency for agonists of this receptor is oxymetazoline > clonidine > epinephrine > norepinephrine > phenylephrine > dopamine > p-synephrine > p-tyramine > serotonin = p-octopamine. For antagonists, the rank order is yohimbine > phentolamine = mianserine > chlorpromazine = spiperone = prazosin > propanolol > alprenolol = pindolol. Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA2A sub-subfamily.
Protein type: GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR
Chromosomal Location of Human Ortholog: 10q25.2
Cellular Component: basolateral plasma membrane; cytoplasm; integral to plasma membrane; plasma membrane; receptor complex
Molecular Function: alpha-1B adrenergic receptor binding; alpha-2C adrenergic receptor binding; alpha2-adrenergic receptor activity; epinephrine binding; heterotrimeric G-protein binding; norepinephrine binding; protein binding; protein heterodimerization activity; protein homodimerization activity; protein kinase binding; thioesterase binding
Biological Process: actin cytoskeleton organization and biogenesis; activation of protein kinase activity; activation of protein kinase B; cell motility; cellular response to hormone stimulus; G-protein coupled receptor protein signaling pathway; glucose homeostasis; intestinal absorption; negative regulation of adenylate cyclase activity; negative regulation of calcium ion transport; negative regulation of calcium ion-dependent exocytosis; negative regulation of cAMP biosynthetic process; negative regulation of insulin secretion; negative regulation of lipid catabolic process; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of cytokine production; positive regulation of epidermal growth factor receptor activity; positive regulation of MAP kinase activity; positive regulation of membrane protein ectodomain proteolysis; positive regulation of potassium ion transport; Ras protein signal transduction; regulation of insulin secretion; Rho protein signal transduction; signal transduction
Research Articles on ADRA2A
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Product Notes
The ADRA2A adra2a (Catalog #AAA420023) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-ADRA2A Antibody reacts with Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's ADRA2A can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Preliminary experiments gave a band at approx 37kDa and a minor band at approx 23kDa in Human Brain lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 49. 0kDa according to NP_000672.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). Immunofluorescence: This product has been successfully used in IF on Mouse primary DRG cultured neurons. Researchers should empirically determine the suitability of the ADRA2A adra2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: TERRPNGLGP ERS. It is sometimes possible for the material contained within the vial of "ADRA2A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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