Rabbit anti-Human ADRA1B Polyclonal Antibody | anti-ADRA1B antibody

ADRA1B, CT (ADRA1B, Alpha-1B adrenergic receptor, Alpha-1B adrenoreceptor) (AP)

Cat. Num. AAA6271893

• Gene Names: ADRA1B; ADRA1; ALPHA1BAR
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ADRA1B; Polyclonal Antibody; CT (ADRA1B; Alpha-1B adrenergic receptor; Alpha-1B adrenoreceptor) (AP); Alpha-1B adrenoreceptor; anti-ADRA1B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-ADRA1B antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ADRA1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 380-409 amino acids from the C-terminal region of human ADRA1B.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(ADRA1B Antibody (C-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the ADRA1B antibody detected the ADRA1B protein (arrow).)

Product Categories/Family for anti-ADRA1B antibody

Antibodies; Receptors

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Pinheiro, A.P., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 153B (5), 1070-1080 (2010): Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Jensen, B.C., et al. Circ Heart Fail 2(6):654-663(2009). Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4501959
• NCBI GeneID: 147
• NCBI Accession #: NP_000670.1
• NCBI GenBank Nucleotide #: NM_000679.3
• UniProt Accession #: P35368; B0LPE1
• Molecular Weight: 56,836 Da
• NCBI Official Full Name: alpha-1B adrenergic receptor
• NCBI Official Synonym Full Names: adrenoceptor alpha 1B
• NCBI Official Symbol: ADRA1B
• NCBI Official Synonym Symbols: ADRA1; ALPHA1BAR
• NCBI Protein Information: alpha-1B adrenergic receptor
• UniProt Protein Name: Alpha-1B adrenergic receptor
• Protein Family: Alpha-1B adrenergic receptor
• UniProt Gene Name: ADRA1B
• UniProt Synonym Gene Names: Alpha-1B adrenoceptor
• UniProt Entry Name: ADA1B_HUMAN

NCBI Description

Alpha-1-adrenergic receptors (alpha-1-ARs) are members of the G protein-coupled receptor superfamily. They activate mitogenic responses and regulate growth and proliferation of many cells. There are 3 alpha-1-AR subtypes: alpha-1A, -1B and -1D, all of which signal through the Gq/11 family of G-proteins and different subtypes show different patterns of activation. This gene encodes alpha-1B-adrenergic receptor, which induces neoplastic transformation when transfected into NIH 3T3 fibroblasts and other cell lines. Thus, this normal cellular gene is identified as a protooncogene. This gene comprises 2 exons and a single large intron of at least 20 kb that interrupts the coding region. [provided by RefSeq, Jul 2008]

Uniprot Description

ADRA1B: a G protein-coupled catecholamine receptor that activates mitogenic responses and regulates growth and proliferation of many cells. There are 3 alpha-1-AR subtypes: alpha-1A, -1B and -1D, all of which signal through the Gq/11 family of G-proteins and different subtypes show different patterns of activation. Can induce neoplastic transformation when transfected into cell lines. Can regulate the phosphorylation status of the STAT3 pathway. Mediates blood pressure and aorta contractile responses induced by alpha-1 agonists. Activates a phosphatidylinositol second messenger system.

Protein type: Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass; GPCR, family 1

Chromosomal Location of Human Ortholog: 5q33.3

Cellular Component: nuclear membrane; integral to plasma membrane; plasma membrane; nucleus

Molecular Function: protein heterodimerization activity; alpha1-adrenergic receptor activity

Biological Process: adult heart development; vasoconstriction of artery involved in baroreceptor response to lowering of systemic arterial blood pressure; positive regulation of the force of heart contraction by epinephrine-norepinephrine; multicellular organismal development; response to amphetamine; response to morphine; locomotory behavior; glucose homeostasis; positive regulation of glycogen catabolic process; G-protein signaling, coupled to cAMP nucleotide second messenger; cell proliferation; G-protein coupled receptor protein signaling pathway; organ growth; positive regulation of MAPKKK cascade; cell-cell signaling; behavioral response to cocaine; regulation of vasoconstriction; visual learning; blood vessel remodeling; cell growth; positive regulation of heart rate by epinephrine-norepinephrine; negative regulation of glycogen catabolic process