Rabbit ADRA1A Polyclonal Antibody | anti-ADRA1A antibody

ADRA1A Polyclonal Antibody

Cat. Num. AAA9128255

• Gene Names: ADRA1A; ADRA1C; ADRA1L1; ALPHA1AAR
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purification
• Synonyms: ADRA1A; Polyclonal Antibody; ADRA1A Polyclonal Antibody; anti-ADRA1A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Sequence Length: 466

• Applicable Applications for anti-ADRA1A antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB 1:500 - 1:2000; IHC 1:50 - 1:200
• Route: Synthetic Peptide
• Immunogen: A synthetic peptide of human ADRA1A
• Preparation and Storage: Store at -20 degree C. Avoid freeze / thaw cycles.; Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using ADRA1A antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 30s.)

Related Product Information for anti-ADRA1A antibody

Alpha-1-adrenergic receptors (alpha-1-ARs) are members of the G protein-coupled receptor superfamily. They activate mitogenic responses and regulate growth and proliferation of many cells. There are 3 alpha-1-AR subtypes: alpha-1A, -1B and -1D, all of which signal through the Gq/11 family of G-proteins and different subtypes show different patterns of activation. This gene encodes alpha-1A-adrenergic receptor. Alternative splicing of this gene generates four transcript variants, which encode four different isoforms with distinct C-termini but having similar ligand binding properties.

NCBI and Uniprot Product Information

• NCBI GI #: 111118992
• NCBI GeneID: 148
• NCBI Accession #: NP_000671.2
• NCBI GenBank Nucleotide #: NM_000680.2
• UniProt Accession #: P35348; O60451; Q13675; Q13729; A8K0I3; B0ZBD1; B0ZBD2; B0ZBD4; B0ZBD5; B0ZBD6; B0ZBD8; B0ZBD9
• Molecular Weight: 51kDa
• NCBI Official Full Name: alpha-1A adrenergic receptor isoform 1
• NCBI Official Synonym Full Names: adrenoceptor alpha 1A
• NCBI Official Symbol: ADRA1A
• NCBI Official Synonym Symbols: ADRA1C; ADRA1L1; ALPHA1AAR
• NCBI Protein Information: alpha-1A adrenergic receptor
• UniProt Protein Name: Alpha-1A adrenergic receptor
• Protein Family: Alpha-1A adrenergic receptor
• UniProt Gene Name: ADRA1A
• UniProt Synonym Gene Names: ADRA1C; Alpha-1A adrenoceptor
• UniProt Entry Name: ADA1A_HUMAN

NCBI Description

Alpha-1-adrenergic receptors (alpha-1-ARs) are members of the G protein-coupled receptor superfamily. They activate mitogenic responses and regulate growth and proliferation of many cells. There are 3 alpha-1-AR subtypes: alpha-1A, -1B and -1D, all of which signal through the Gq/11 family of G-proteins and different subtypes show different patterns of activation. This gene encodes alpha-1A-adrenergic receptor. Alternative splicing of this gene generates four transcript variants, which encode four different isoforms with distinct C-termini but having similar ligand binding properties. [provided by RefSeq, Jul 2008]

Uniprot Description

ADRA1A: This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol- calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine(PE)-stimulated ERK signaling in cardiac myocytes. Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA1A sub-subfamily. 9 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 1; Membrane protein, integral

Chromosomal Location of Human Ortholog: 8p21.2

Cellular Component: nuclear membrane; integral to plasma membrane; T-tubule; plasma membrane; nucleus; Z disc

Molecular Function: protein heterodimerization activity; alpha1-adrenergic receptor activity

Biological Process: adult heart development; response to drug; apoptosis; positive regulation of the force of heart contraction by epinephrine-norepinephrine; positive regulation of synaptic transmission, GABAergic; response to hormone stimulus; positive regulation of systemic arterial blood pressure; norepinephrine-epinephrine vasoconstriction involved in regulation of systemic arterial blood pressure; signal transduction; micturition; G-protein coupled receptor protein signaling pathway; negative regulation of cell proliferation; negative regulation of heart rate in baroreceptor response to increased systemic arterial blood pressure; organ growth; elevation of cytosolic calcium ion concentration; smooth muscle contraction; cell-cell signaling; negative regulation of Rho protein signal transduction; positive regulation of MAPKKK cascade; phospholipase C activation; positive regulation of vasoconstriction; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of smooth muscle contraction; positive regulation of action potential; cell growth; positive regulation of heart rate by epinephrine-norepinephrine; aging