Rabbit anti-Human ADORA2A Polyclonal Antibody | anti-ADORA2A antibody

ADORA2A, ID (ADORA2A, ADORA2, Adenosine receptor A2a) (FITC)

Cat. Num. AAA6271863

• Gene Names: ADORA2A; A2aR; RDC8; ADORA2
• Reactivity: Human
• Applications: Immunofluorescence, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ADORA2A; Polyclonal Antibody; ID (ADORA2A; ADORA2; Adenosine receptor A2a) (FITC); Adenosine receptor A2a; anti-ADORA2A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-ADORA2A antibody: Immunofluorescence (IF), FLISA, Western Blot (WB)
• Application Notes: IF: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: ADORA2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 280-309 amino acids from the Central region of human ADORA2A.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ADORA2A Antibody (Center) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the ADORA2A antibody detected the ADORA2A protein (arrow).)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of ADORA2A Antibody (Center) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-ADORA2A antibody

ADORA2A is a protein which is one of several receptor subtypes for adenosine. The activity of the encoded protein, a G-protein coupled receptor family member, is mediated by G proteins which activate adenylyl cyclase. This protein is abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine.

Product Categories/Family for anti-ADORA2A antibody

Antibodies; Receptors

References

Buira, S.P., et al. J. Neurochem. 115(1):283-295(2010). Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Rogers, P.J., et al. Neuropsychopharmacology 35(9):1973-1983(2010). Tebano, M.T., et al. ScientificWorldJournal 10, 1768-1782 (2010): Kobayashi, H., et al. Behav Brain Funct 6, 50 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 5921992
• NCBI GeneID: 135
• NCBI Accession #: NP_000666.2
• NCBI GenBank Nucleotide #: NM_000675.5
• UniProt Accession #: P29274; B2R7E0
• Molecular Weight: 44,707 Da
• NCBI Official Full Name: adenosine receptor A2a
• NCBI Official Synonym Full Names: adenosine A2a receptor
• NCBI Official Symbol: ADORA2A
• NCBI Official Synonym Symbols: A2aR; RDC8; ADORA2
• NCBI Protein Information: adenosine receptor A2a; adenosine receptor subtype A2a
• UniProt Protein Name: Adenosine receptor A2a
• Protein Family: Adenosine receptor
• UniProt Gene Name: ADORA2A
• UniProt Synonym Gene Names: ADORA2
• UniProt Entry Name: AA2AR_HUMAN

NCBI Description

This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor (GPCR) superfamily, which is subdivided into classes and subtypes. The receptors are seven-pass transmembrane proteins that respond to extracellular cues and activate intracellular signal transduction pathways. This protein, an adenosine receptor of A2A subtype, uses adenosine as the preferred endogenous agonist and preferentially interacts with the G(s) and G(olf) family of G proteins to increase intracellular cAMP levels. It plays an important role in many biological functions, such as cardiac rhythm and circulation, cerebral and renal blood flow, immune function, pain regulation, and sleep. It has been implicated in pathophysiological conditions such as inflammatory diseases and neurodegenerative disorders. Alternative splicing results in multiple transcript variants. A read-through transcript composed of the upstream SPECC1L (sperm antigen with calponin homology and coiled-coil domains 1-like) and ADORA2A (adenosine A2a receptor) gene sequence has been identified, but it is thought to be non-coding. [provided by RefSeq, Jun 2013]

Uniprot Description

ADORA2A: one of several receptor subtypes for adenosine. A G-protein coupled receptor. Activation is mediated by G proteins which activate adenylyl cyclase. Abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine.

Protein type: Membrane protein, integral; Receptor, GPCR; GPCR, family 1; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 22q11.23

Cellular Component: membrane; integral to plasma membrane; plasma membrane

Molecular Function: identical protein binding; protein binding; enzyme binding; adenosine receptor activity, G-protein coupled

Biological Process: central nervous system development; nerve growth factor receptor signaling pathway; blood circulation; apoptosis; adenylate cyclase activation; phagocytosis; G-protein signaling, coupled to cAMP nucleotide second messenger; cell-cell signaling; sensory perception; cellular defense response; cAMP biosynthetic process; inflammatory response; blood coagulation; adenosine receptor signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway