Rabbit ADO1 Polyclonal Antibody | anti-ADO1 antibody
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ADO1 Polyclonal Antibody
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH7.4
2. 1mL preimmune serum (negative control)
3. 10mg rabbit polyclonal antibody purified by Protein A/G.
2. Antibody titer > 1: 64,000 confirmed by ELISA
3. Western Blot positive for the immunogen protein (Not applicable for synthetic peptide).
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NCBI and Uniprot Product Information
Uniprot Description
Function: Component of an E3 ubiquitin ligase complex that plays a central role in blue light-dependent circadian cycles. Acts as a blue light photoreceptor, due to the presence of FMN, that mediates light-regulated protein degradation of critical clock components by targeting them to the proteasome complex. The SCF(ADO1) E3 ubiquitin ligase complex is involved in the regulation of circadian clock-dependent processes including the transition to flowering time, hypocotyl elongation, cotyledons and leaf movement rhythms. APRR1/TOC1 and APRR5, but not 'GIGANTEA', are proteolytic substrates of this ubiquitin ligase complex. Blue light enhances cooperative stabilization of 'GIGANTEA' and ADO1/ZTL, leading to amplification and sharpening of the expression profile of APRR1/TOC1. ADO1/ZTL interacts with ADO3, preventing the interaction of ADO3 with CDF1. Ref.1 Ref.2 Ref.3 Ref.4 Ref.12 Ref.13 Ref.14 Ref.15 Ref.16
Pathway: Protein modification; protein ubiquitination.
Subunit structure: Interacts with NFXL2. Interacts (via N-terminus) with GI and (via Kelch repeats) with ADO3. Component of an E3 ubiquitin ligase SCF(ADO1) complex composed of SKP1A/ASK1 (or SKP1B/ASK2), CUL1, RBX1 and ADO1. Also interacts with SKP1D/ASK4, SKP1K/ASK11, CRY1, PHYB, APRR1 and APRR5, and probably with SKP1N/ASK14 and SKP1S/ASK19. Ref.4 Ref.9 Ref.11 Ref.13 Ref.14 Ref.15 Ref.16 Ref.17
Subcellular location: Nucleus. Cytoplasm. Note: Nuclear after 9 hours of illumination (afternoon of long days). Cytoplasmic when plant have been subsequently grown 16 hours in light and 5 hours in dark (early morning of long days). Ref.3 Ref.15 Ref.16
Tissue specificity: Ubiquitously expressed with higher levels in cotyledons and leaves. Ref.2 Ref.3
Developmental stage: Mainly present during the light phase, and degraded in a proteasome-dependent manner in dark (at protein level).
Induction: Not regulated at the transcript level, but circadian-regulation at the protein level with a peak at the end of the subjective day. Ref.1 Ref.3 Ref.10 Ref.15 Ref.17
Post-translational modification: May be ubiquitinated. Degraded in a proteasome-dependent manner.FMN binds covalently to cysteine after exposure to blue light and is reversed in the dark
By similarity.
Disruption phenotype: Accumulation of ADO3 protein during the morning period and early flowering time. Ref.16
Miscellaneous: 'Zeitlupe' means slow motion in German.'Adagio' means slowly in Italian.
Sequence similarities: Belongs to the ADAGIO family.Contains 1 F-box domain.Contains 5 Kelch repeats.Contains 1 PAC (PAS-associated C-terminal) domain.Contains 1 PAS (PER-ARNT-SIM) domain.
Sequence caution: The sequence AAK64006.1 differs from that shown. Reason: Frameshift at positions 462 and 467.
Research Articles on ADO1
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Product Notes
The ADO1 ado1 (Catalog #AAA9005757) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ADO1 Polyclonal Antibody reacts with Arabidopsis thaliana (Mouse-ear cress) and may cross-react with other species as described in the data sheet. AAA Biotech's ADO1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), WB (Western Blot) (ensure identification of antigen). Researchers should empirically determine the suitability of the ADO1 ado1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ADO1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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