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Western Blot (WB) (Western blot analysis on LOVO cell lysate using ADNP Antibody, The lane on the left is treated with the antigen-specific peptide.)

Rabbit anti-Human, Mouse ADNP Polyclonal Antibody | anti-ADNP antibody

ADNP Antibody

Gene Names
ADNP; ADNP1; HVDAS; MRD28
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
ADNP; Polyclonal Antibody; ADNP Antibody; Activity dependent neuroprotective protein; Activity dependent neuroprotector; Activity-dependent neuroprotective protein; Activity-dependent neuroprotector homeobox protein; Adnp; ADNP_HUMAN; KIAA0784; anti-ADNP antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
ADNP antibody detects endogenous levels of ADNP
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
1102
Applicable Applications for anti-ADNP antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide derived from human ADNP
Subcellular Location
Nucleus
Tissue Specificity
Widely expressed. Strong expression in heart, skeletal muscle, kidney and placenta. In brain, expression is stronger in the cerebellum and cortex regions. No expression detected in the colon. Strong increase of expression in colon and breast cancer tissues.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Similarity
Pig (92%), Bovine (83%), Horse (92%), Sheep (83%), Rabbit (100%), Dog (92%), Chicken (83%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis on LOVO cell lysate using ADNP Antibody, The lane on the left is treated with the antigen-specific peptide.)

Western Blot (WB) (Western blot analysis on LOVO cell lysate using ADNP Antibody, The lane on the left is treated with the antigen-specific peptide.)

Immunofluorescene (IF)

(MBS9600326 staining LOVO by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

Immunofluorescene (IF) (MBS9600326 staining LOVO by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Related Product Information for anti-ADNP antibody
Description: ADNP Potential transcription factor. May mediate some of the neuroprotective peptide VIP-associated effects involving normal growth and cancer proliferation.
Function: Potential transcription factor. May mediate some of the neuroprotective peptide VIP-associated effects involving normal growth and cancer proliferation.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 124 kDa
Predicted: 124 kDa
NCBI Official Full Name
activity-dependent neuroprotector homeobox protein
NCBI Official Synonym Full Names
activity dependent neuroprotector homeobox
NCBI Official Symbol
ADNP
NCBI Official Synonym Symbols
ADNP1; HVDAS; MRD28
NCBI Protein Information
activity-dependent neuroprotector homeobox protein
UniProt Protein Name
Activity-dependent neuroprotector homeobox protein
Protein Family
UniProt Gene Name
ADNP
UniProt Synonym Gene Names
ADNP1; KIAA0784

NCBI Description

Vasoactive intestinal peptide is a neuroprotective factor that has a stimulatory effect on the growth of some tumor cells and an inhibitory effect on others. This gene encodes a protein that is upregulated by vasoactive intestinal peptide and may be involved in its stimulatory effect on certain tumor cells. The encoded protein contains one homeobox and nine zinc finger domains, suggesting that it functions as a transcription factor. This gene is also upregulated in normal proliferative tissues. Finally, the encoded protein may increase the viability of certain cell types through modulation of p53 activity. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

Potential transcription factor. May mediate some of the neuroprotective peptide VIP-associated effects involving normal growth and cancer proliferation.

Research Articles on ADNP

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Product Notes

The ADNP adnp (Catalog #AAA9600326) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ADNP Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ADNP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:2000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the ADNP adnp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ADNP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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