Rabbit anti-Human ADIPOR1 Polyclonal Antibody | anti-ADIPOR1 antibody

ADIPOR1, CT (Adiponectin Receptor Protein 1, Progestin And adipoQ Receptor Family Member I, CGI-45, PAQR1, TESBP1A) (MaxLight 650)

Cat. Num. AAA6461716

• Gene Names: ADIPOR1; CGI45; PAQR1; ACDCR1; CGI-45; TESBP1A
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: ADIPOR1; Polyclonal Antibody; CT (Adiponectin Receptor Protein 1; Progestin And adipoQ Receptor Family Member I; CGI-45; PAQR1; TESBP1A) (MaxLight 650); anti-ADIPOR1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ADIPOR1.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-ADIPOR1 antibody: Immunohistochemistry (IHC) Paraffin, FLISA, Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 305-331 in the C-terminal region of human ADIPOR1. Species sequence homology: mouse
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in Y79 cell line lysates (35ug/lane) usingMBS631276. ADIPOR1 (arrow) was detected usingMBS631276.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human hepatocarcinoma using AA0886-75G, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS631276 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of widr cells using MBS631276 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-ADIPOR1 antibody

Antibodies; Receptors

References

1. Civitarese, A.E., et.al., Diabetologia 47(5), 816-820 (2004). 2. Wang, H., et.al., Diabetes 53(8), 2132-2136 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 21361519
• NCBI GeneID: 51094
• NCBI Accession #: NP_057083.2
• NCBI GenBank Nucleotide #: NM_015999.5
• UniProt Accession #: Q96A54; Q53HS7; Q53YY6; Q9Y360; B3KMB0
• Molecular Weight: 42,616 Da
• NCBI Official Full Name: adiponectin receptor protein 1
• NCBI Official Synonym Full Names: adiponectin receptor 1
• NCBI Official Symbol: ADIPOR1
• NCBI Official Synonym Symbols: CGI45; PAQR1; ACDCR1; CGI-45; TESBP1A
• NCBI Protein Information: adiponectin receptor protein 1
• UniProt Protein Name: Adiponectin receptor protein 1
• Protein Family: Adiponectin receptor protein
• UniProt Gene Name: ADIPOR1
• UniProt Synonym Gene Names: PAQR1; TESBP1A
• UniProt Entry Name: ADR1_HUMAN

NCBI Description

This gene encodes a protein which acts as a receptor for adiponectin, a hormone secreted by adipocytes which regulates fatty acid catabolism and glucose levels. Binding of adiponectin to the encoded protein results in activation of an AMP-activated kinase signaling pathway which affects levels of fatty acid oxidation and insulin sensitivity. A pseudogene of this gene is located on chromosome 14. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2014]

Uniprot Description

ADIPOR1: Receptor for globular and full-length adiponectin (APM1), an essential hormone secreted by adipocytes that acts as an antidiabetic. Probably involved in metabolic pathways that regulate lipid metabolism such as fatty acid oxidation. Mediates increased AMPK, PPARA ligand activity, fatty acid oxidation and glucose uptake by adiponectin. Has some high-affinity receptor for globular adiponectin but low-affinity receptor for full-length adiponectin. Belongs to the ADIPOR family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: membrane; plasma membrane; integral to membrane

Molecular Function: adiponectin binding; identical protein binding; hormone binding; protein heterodimerization activity; receptor activity; protein kinase binding

Biological Process: hormone-mediated signaling; adiponectin-mediated signaling pathway; positive regulation of JAK-STAT cascade; fatty acid oxidation; negative regulation of cell growth; positive regulation of insulin receptor signaling pathway; leptin-mediated signaling pathway