Microtubule Associated Protein 2 (MAP2) Recombinant Protein | MAP2 recombinant protein

Recombinant Microtubule Associated Protein 2 (MAP2)

Cat. Num. AAA2011341

• Gene Names: Map2; MAP2R; Mtap2
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Microtubule Associated Protein 2 (MAP2); Recombinant Microtubule Associated Protein 2 (MAP2); MAP2 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-TV SRDEVRRKKA VYKKAELAKE SEVQAHSPSR KLILKPAIKY TRPTHLSCVK RKTTATSGES AQAPSAFKQA KDKVTDGITK SPEKRSSLPR PSSILPPRRG VSGDREENSF SLNSSISSAR RTTRSEPIRR AGKSGTSTPT TPGSTAITPG TPPSYSSRTP GTPGTPSYPR TPGTPKSGIL VPSEKKVAII RTPPKSPATP KQLRLINQPL PDLKNVKSKI GSTDNIKYQP KGGQVQIVTK KIDLSHVTSK CGSLKNIR
• Sequence Length: 1861

• Applicable Applications for MAP2 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Thr1459~Arg1718 (Accession # P15146) with two N-terminal Tags, His-tag and GST-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 547890
• NCBI GeneID: 25595
• UniProt Accession #: P15146
• Molecular Weight: 60.0kDa
• NCBI Official Full Name: Microtubule-associated protein 2
• NCBI Official Synonym Full Names: microtubule-associated protein 2
• NCBI Official Symbol: Map2
• NCBI Official Synonym Symbols: MAP2R; Mtap2
• NCBI Protein Information: microtubule-associated protein 2; MAP-2
• UniProt Protein Name: Microtubule-associated protein 2
• Protein Family: Microtubule-associated protein
• UniProt Gene Name: Map2
• UniProt Synonym Gene Names: Mtap2; MAP-2
• UniProt Entry Name: MTAP2_RAT

NCBI Description

regulates and organizes microtubules; may serve as a scaffolding protein for neuromodulatory activities [RGD, Feb 2006]

Uniprot Description

MAP2: a member of the microtubule-associated protein (MAP) family. Its exact function is unknown but MAPs appear to stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. Expressed at high levels in neural tissue and the prostate. Four alternatively spliced transcript variants have been reported.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Cellular Component: microtubule; neuron projection; protein complex; smooth endoplasmic reticulum; rough endoplasmic reticulum; cell soma; postsynaptic density; dendrite; cytoplasm; nucleolus; dendritic shaft; intracellular

Molecular Function: calmodulin binding; protein binding; microtubule binding

Biological Process: axonogenesis; dendrite morphogenesis; dendrite development; response to progesterone stimulus; microtubule bundle formation; neurite development; response to estradiol stimulus; central nervous system neuron development