Testing Data
(A549 cells were stained with MBS605136 (filled histogram) or isotype control antibody (open histogram), followed by APC-conjugated anti-mouse antibody.)
Mouse anti-Human DMC Monoclonal Antibody | anti-DYM antibody
DMC (Dendritic cell and Monocyte Chemokine-like Protein, VEGF Co-regulated Chemokine 1, VCC1)
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Dilution: Flow Cytometry: 25ug/ml. 10ul labels 1-5x10e5 A549 cells in a total reaction volume 200ul. For intracellular staining, cells must first be fixed and permeabilized using 4% paraformaldehyde, 0.1% saponin in PBS.
Immunocytochemistry: 10ug/ml detects DMC in A549 cells. Cells were fixed with PBS, 4% paraformaldehyde and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-100, 1% BSA. After blocking, cells were incubated with diluted D8074-90B followed by NL557-coupled anti-mouse IgG in the dark. Between each step, cells were washed with PBS, BSA.
Direct ELISA: 0.5-1ug/ml. The detection limit is ~10ng/well.
Western Blot: 1-2ug/ml. Using a colorimetric detection system, the detection limit is ~25ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold.
Testing Data
(A549 cells were stained with MBS605136 (filled histogram) or isotype control antibody (open histogram), followed by APC-conjugated anti-mouse antibody.)
Testing Data
(A549 cells were stained with MBS605136 (filled histogram) or isotype control antibody (open histogram), followed by APC-conjugated anti-mouse antibody.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein which is necessary for normal skeletal development and brain function. Mutations in this gene are associated with two types of recessive osteochondrodysplasia, Dyggve-Melchior-Clausen (DMC) dysplasia and Smith-McCort (SMC) dysplasia, which involve both skeletal defects and mental retardation. [provided by RefSeq, Jul 2008]
Uniprot Description
DYM: Necessary for correct organization of Golgi apparatus. Involved in bone development. Defects in DYM are the cause of Dyggve-Melchior-Clausen syndrome (DMC). DMC is a rare autosomal recessive disorder characterized by short trunk dwarfism, microcephaly and psychomotor retardation. Electron microscopic study of cutaneous cells of affected patients shows dilated rough endoplasmic reticulum, enlarged and aberrant vacuoles and numerous vesicles. DMC is progressive. Defects in DYM are the cause of Smith-McCort dysplasia (SMC). SMC is a rare autosomal recessive osteochondrodysplasia characterized by short limbs and trunk with barrel-shaped chest. The radiographic phenotype includes platyspondyly, generalized abnormalities of the epiphyses and metaphyses, and a distinctive lacy appearance of the iliac crest, features identical to those of Dyggve-Melchior-Clausen syndrome. Belongs to the dymeclin family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 18q21.1
Cellular Component: Golgi apparatus; cytoplasm
Molecular Function: protein binding; enzyme binding
Biological Process: Golgi organization and biogenesis
Disease: Dyggve-melchior-clausen Disease; Smith-mccort Dysplasia 1
Research Articles on DYM
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Product Notes
The DYM dym (Catalog #AAA605136) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The DMC (Dendritic cell and Monocyte Chemokine-like Protein, VEGF Co-regulated Chemokine 1, VCC1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DMC can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS). Suitable for use in Flow Cytometry, Immunocytochemistry, ELISA and Western Blot. Dilution: Flow Cytometry: 25ug/ml. 10ul labels 1-5x10e5 A549 cells in a total reaction volume 200ul. For intracellular staining, cells must first be fixed and permeabilized using 4% paraformaldehyde, 0.1% saponin in PBS. Immunocytochemistry: 10ug/ml detects DMC in A549 cells. Cells were fixed with PBS, 4% paraformaldehyde and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-100, 1% BSA. After blocking, cells were incubated with diluted D8074-90B followed by NL557-coupled anti-mouse IgG in the dark. Between each step, cells were washed with PBS, BSA. Direct ELISA: 0.5-1ug/ml. The detection limit is ~10ng/well. Western Blot: 1-2ug/ml. Using a colorimetric detection system, the detection limit is ~25ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Researchers should empirically determine the suitability of the DYM dym for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DMC, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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