Rabbit anti-Human ADH7 Polyclonal Antibody | anti-ADH7 antibody

ADH7, CT (ADH7, Alcohol dehydrogenase class 4 mu/sigma chain, Alcohol dehydrogenase class IV mu/sigma chain, Gastric alcohol dehydrogenase, Retinol dehydrogenase) (FITC)

Cat. Num. AAA6271775

• Gene Names: ADH7; ADH4
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ADH7; Polyclonal Antibody; CT (ADH7; Alcohol dehydrogenase class 4 mu/sigma chain; Alcohol dehydrogenase class IV mu/sigma chain; Gastric alcohol dehydrogenase; Retinol dehydrogenase) (FITC); Retinol dehydrogenase; anti-ADH7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-ADH7 antibody: Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IF: 1:10-50; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: ADH7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 325~354 amino acids from the C-terminal region of human ADH7 Antibody (C-Term).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ADH7 Antibody (C-Term) western blot analysis in Jurkat,CEM,NCI-H460,K562,MDA-MB435 cell line lysates (35ug/lane).This demonstrates the ADH7 antibody detected the ADH7 protein (arrow).)

Immunohistochemistry (IHC)

(ADH7 Antibody (C-Term) IHC analysis in formalin fixed and paraffin embedded lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ADH7 Antibody (C-Term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(ADH7 Antibody (C-Term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of ADH7 Antibody (C-Term) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-ADH7 antibody

This gene encodes class IV alcohol dehydrogenase 7 mu or sigma subunit, which is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. The enzyme encoded by this gene is inefficient in ethanol oxidation, but is the most active as a retinol dehydrogenase; thus it may participate in the synthesis of retinoic acid, a hormone important for cellular differentiation. The expression of this gene is much more abundant in stomach than liver, thus differing from the other known gene family members.

Product Categories/Family for anti-ADH7 antibody

Antibodies; Enzymes; Dehydrogenase

References

Kedishvili, N.Y., et al. J. Biol. Chem. 270(8):3625-3630(1995) Cheung, B., et al. Alcohol. Clin. Exp. Res. 19(1):185-186(1995) Farres, J., et al. Eur. J. Biochem. 224(2):549-557(1994) Pares, X., et al. FEBS Lett. 303(1):69-72(1992).

NCBI and Uniprot Product Information

• NCBI GI #: 71743840
• NCBI GeneID: 131
• NCBI Accession #: NP_000664.2
• NCBI GenBank Nucleotide #: NM_000673.4
• UniProt Accession #: P40394; Q13713; A2RRB6; A8MVN9; B2R760; B4DWV6
• Molecular Weight: 41,481 Da
• NCBI Official Full Name: alcohol dehydrogenase class 4 mu/sigma chain isoform 2
• NCBI Official Synonym Full Names: alcohol dehydrogenase 7 (class IV), mu or sigma polypeptide
• NCBI Official Symbol: ADH7
• NCBI Official Synonym Symbols: ADH4
• NCBI Protein Information: alcohol dehydrogenase class 4 mu/sigma chain; retinol dehydrogenase; alcohol dehydrogenase-7; alcohol dehydrogenase VII; gastric alcohol dehydrogenase; class IV sigma-1 alcohol dehydrogenase; class IV sigmasigma alcohol dehydrogenase; alcohol dehydrogenas
• UniProt Protein Name: Alcohol dehydrogenase class 4 mu/sigma chain
• Protein Family: Alcohol dehydrogenase
• UniProt Gene Name: ADH7
• UniProt Entry Name: ADH7_HUMAN

NCBI Description

This gene encodes class IV alcohol dehydrogenase 7 mu or sigma subunit, which is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. The enzyme encoded by this gene is inefficient in ethanol oxidation, but is the most active as a retinol dehydrogenase; thus it may participate in the synthesis of retinoic acid, a hormone important for cellular differentiation. The expression of this gene is much more abundant in stomach than liver, thus differing from the other known gene family members. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]

Uniprot Description

ADH7: Could function in retinol oxidation for the synthesis of retinoic acid, a hormone important for cellular differentiation. Medium-chain (octanol) and aromatic (m-nitrobenzaldehyde) compounds are the best substrates. Ethanol is not a good substrate but at the high ethanol concentrations reached in the digestive tract, it plays a role in the ethanol oxidation and contributes to the first pass ethanol metabolism. Belongs to the zinc-containing alcohol dehydrogenase family. Class-IV subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Xenobiotic Metabolism - drug metabolism - cytochrome P450; EC 1.1.1.1; Amino Acid Metabolism - tyrosine; Lipid Metabolism - fatty acid; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Oxidoreductase; Xenobiotic Metabolism - metabolism by cytochrome P450; Cofactor and Vitamin Metabolism - retinol

Chromosomal Location of Human Ortholog: 4q23-q24

Cellular Component: extracellular region; cytosol

Molecular Function: receptor antagonist activity; zinc ion binding; aldehyde oxidase activity; alcohol dehydrogenase activity; alcohol dehydrogenase activity, zinc-dependent; ethanol binding; retinol binding; retinol dehydrogenase activity

Biological Process: response to ethanol; ethanol catabolic process; response to bacterium; retinol metabolic process; xenobiotic metabolic process; retinoic acid metabolic process; retinoid metabolic process; ethanol oxidation; fatty acid omega-oxidation