Rabbit anti-Human ADH7 Polyclonal Antibody | anti-ADH7 antibody

ADH7 (Alcohol Dehydrogenase Class 4 mu/sigma Chain, Alcohol Dehydrogenase Class IV mu/sigma Chain, Gastric Alcohol Dehydrogenase, Retinol Dehydrogenase) (HRP)

Cat. Num. AAA6368916

• Gene Names: ADH7; ADH4
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ADH7; Polyclonal Antibody; ADH7 (Alcohol Dehydrogenase Class 4 mu/sigma Chain; Alcohol Dehydrogenase Class IV mu/sigma Chain; Gastric Alcohol Dehydrogenase; Retinol Dehydrogenase) (HRP); anti-ADH7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ADH7.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-ADH7 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human ADH7, aa1-386 (NP_000664.2).
• Immunogen Sequence: MFAEIQIQDKDRMGTAGKVIKCKAAVLWEQKQPFSIEEIEVAPPKTKEVRIKILATGICRTDDHVIKGTMVSKFPVIVGHEATGIVESIGEGVTTVKPGDKVIPLFLPQCRECNACRNPDGNLCIRSDITGRGVLADGTTRFTCKGKPVHHFMNTSTFTEYTVVDESSVAKIDDAAPPEKVCLIGCGFSTGYGAAVKTGKVKPGSTCVVFGLGGVGLSVIMGCKSAGASRIIGIDLNKDKFEKAMAVGATECISPKDSTKPISEVLSEMTGNNVGYTFEVIGHLETMIDALASCHMNYGTSVVVGVPPSAKMLTYDPMLLFTGRTWKGCVFGGLKSRDDVPKLVTEFLAKKFDLDQLITHVLPFKKISEGFELLNSGQSIRTVLTF
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of ADH7 expression in transfected 293T cell line by ADH7 polyclonal antibody. Lane 1: ADH7 transfected lysate (41.5kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-ADH7 antibody

This gene encodes class IV alcohol dehydrogenase 7 mu or sigma subunit, which is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. The enzyme encoded by this gene is inefficient in ethanol oxidation, but is the most active as a retinol dehydrogenase; thus it may participate in the synthesis of retinoic acid, a hormone important for cellular differentiation. The expression of this gene is much more abundant in stomach than liver, thus differing from the other known gene family members.

Product Categories/Family for anti-ADH7 antibody

Antibodies; Enzymes; Dehydrogenase

NCBI and Uniprot Product Information

• NCBI GI #: 71743840
• NCBI GeneID: 131
• NCBI Accession #: NP_000664.2
• NCBI GenBank Nucleotide #: NM_000673.3
• UniProt Accession #: P40394; Q13713; A2RRB6; A8MVN9; B2R760; B4DWV6
• Molecular Weight: 41,481 Da
• NCBI Official Full Name: alcohol dehydrogenase class 4 mu/sigma chain isoform 2
• NCBI Official Synonym Full Names: alcohol dehydrogenase 7 (class IV), mu or sigma polypeptide
• NCBI Official Symbol: ADH7
• NCBI Official Synonym Symbols: ADH4
• NCBI Protein Information: alcohol dehydrogenase class 4 mu/sigma chain; retinol dehydrogenase; alcohol dehydrogenase-7; alcohol dehydrogenase VII; gastric alcohol dehydrogenase; class IV sigma-1 alcohol dehydrogenase; class IV sigmasigma alcohol dehydrogenase; alcohol dehydrogenas
• UniProt Protein Name: Alcohol dehydrogenase class 4 mu/sigma chain
• Protein Family: Alcohol dehydrogenase
• UniProt Gene Name: ADH7
• UniProt Entry Name: ADH7_HUMAN

NCBI Description

This gene encodes class IV alcohol dehydrogenase 7 mu or sigma subunit, which is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. The enzyme encoded by this gene is inefficient in ethanol oxidation, but is the most active as a retinol dehydrogenase; thus it may participate in the synthesis of retinoic acid, a hormone important for cellular differentiation. The expression of this gene is much more abundant in stomach than liver, thus differing from the other known gene family members. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]

Uniprot Description

ADH7: Could function in retinol oxidation for the synthesis of retinoic acid, a hormone important for cellular differentiation. Medium-chain (octanol) and aromatic (m-nitrobenzaldehyde) compounds are the best substrates. Ethanol is not a good substrate but at the high ethanol concentrations reached in the digestive tract, it plays a role in the ethanol oxidation and contributes to the first pass ethanol metabolism. Belongs to the zinc-containing alcohol dehydrogenase family. Class-IV subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Xenobiotic Metabolism - drug metabolism - cytochrome P450; EC 1.1.1.1; Amino Acid Metabolism - tyrosine; Lipid Metabolism - fatty acid; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Oxidoreductase; Xenobiotic Metabolism - metabolism by cytochrome P450; Cofactor and Vitamin Metabolism - retinol

Chromosomal Location of Human Ortholog: 4q23-q24

Cellular Component: extracellular region; cytosol

Molecular Function: receptor antagonist activity; zinc ion binding; aldehyde oxidase activity; alcohol dehydrogenase activity; alcohol dehydrogenase activity, zinc-dependent; ethanol binding; retinol binding; retinol dehydrogenase activity

Biological Process: response to ethanol; ethanol catabolic process; response to bacterium; retinol metabolic process; xenobiotic metabolic process; retinoic acid metabolic process; retinoid metabolic process; ethanol oxidation; fatty acid omega-oxidation