Rabbit anti-Human Adenosine Deaminase (ADA) Polyclonal Antibody | anti-ADA antibody

Polyclonal Antibody to Adenosine Deaminase (ADA)

Cat. Num. AAA2007007

• Reactivity: Human
• Applications: Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Synonyms: Adenosine Deaminase (ADA); Polyclonal Antibody; Polyclonal Antibody to Adenosine Deaminase (ADA); anti-ADA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
• Concentration: 0.5mg/ml (varies by lot)

• Applicable Applications for anti-ADA antibody: WB; IHC; ICC; IP.
• Application Notes: Western blotting: 0.5-2 ug/mL;1:330-1300; Immunohistochemistry: 5-20 ug/mL;1:33-130; Immunocytochemistry: 5-20 ug/mL;1:33-130; ; Optimal working dilutions must be determined by end user.
• Immunogen: Recombinant ADA (Lys11~Val280) expressed in E.coli.
• Cross Reactivity: Human, Mouse
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2054688
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles.; Store at 4ºC for frequent use.; Aliquot and store at -20ºC for 12 months.; ; Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot: Sample: Mouse Placenta lysate; Primary Ab: 1ug/ml Rabbit Anti-Human ADA Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Stomach Tissue))

NCBI and Uniprot Product Information

• NCBI GI #: 47078295
• NCBI GeneID: 100
• NCBI Accession #: NP_000013.2
• NCBI GenBank Nucleotide #: NM_000022.3
• UniProt Accession #: P00813; Q53F92; Q6LA59
• NCBI Official Full Name: adenosine deaminase isoform 1
• NCBI Official Synonym Full Names: adenosine deaminase
• NCBI Official Symbol: ADA
• NCBI Protein Information: adenosine deaminase
• UniProt Protein Name: Adenosine deaminase
• Protein Family: Regulatory protein
• UniProt Gene Name: ADA
• UniProt Synonym Gene Names: ADA1

NCBI Description

This gene encodes an enzyme that catalyzes the hydrolysis of adenosine to inosine. Various mutations have been described for this gene and have been linked to human diseases. Deficiency in this enzyme causes a form of severe combined immunodeficiency disease (SCID), in which there is dysfunction of both B and T lymphocytes with impaired cellular immunity and decreased production of immunoglobulins, whereas elevated levels of this enzyme have been associated with congenital hemolytic anemia. [provided by RefSeq, Jul 2008]

Uniprot Description

ADA: a enzyme that converts adenosine + H2O into inosine + NH3. Found in all tissues, occurs in large amounts in T-lymphocytes and, at the time of weaning, in gastrointestinal tissues. Genetic ADA deficiencies are a cause of autosomal recessive severe combined immuno-deficiency (SCID). Hereditary hemolytic anemia is caused by expression levels in erythrocytes 50-70 times greater than the norm.

Protein type: EC 3.5.4.4; Hydrolase; Nucleotide Metabolism - purine

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: cell junction; cell soma; cell surface; cytosol; dendrite cytoplasm; external side of plasma membrane; extracellular space; lysosome; membrane

Molecular Function: adenosine deaminase activity; protein binding; purine nucleoside binding; zinc ion binding

Biological Process: adenosine catabolic process; aging; alveolus development; cell adhesion; dATP catabolic process; deoxyadenosine catabolic process; embryonic gut development; germinal center B cell differentiation; histamine secretion; hypoxanthine salvage; inosine biosynthetic process; liver development; negative regulation of adenosine receptor signaling pathway; negative regulation of circadian sleep/wake cycle, non-REM sleep; negative regulation of inflammatory response; negative regulation of leukocyte migration; negative regulation of mature B cell apoptosis; Peyer's patch development; placenta development; positive regulation of alpha-beta T cell differentiation; positive regulation of B cell proliferation; positive regulation of calcium-mediated signaling; positive regulation of germinal center formation; positive regulation of heart rate; positive regulation of smooth muscle contraction; positive regulation of T cell differentiation in thymus; positive regulation of T cell receptor signaling pathway; purine nucleotide salvage; purine ribonucleoside monophosphate biosynthetic process; purine-containing compound salvage; regulation of cell-cell adhesion mediated by integrin; response to drug; response to hydrogen peroxide; response to hypoxia; response to morphine; response to vitamin E; T cell activation; trophectodermal cell differentiation; xanthine biosynthetic process

Disease: Severe Combined Immunodeficiency, Autosomal Recessive, T Cell-negative, B Cell-negative, Nk Cell-negative, Due To Adenosine Deaminase Deficiency