anti-Rat Adenosine A1 Receptor Polyclonal Antibody | anti-ADORA1 antibody
Anti - Adenosine A1 Receptor (A1R)
Gene Names
ADORA1; RDC7
Reactivity
Rat
Applications
ELISA, Western Blot
Purity
Serum
Synonyms
Adenosine A1 Receptor; Polyclonal Antibody; Anti - Adenosine A1 Receptor (A1R); Adenosine A1 Receptor (A1R); anti-ADORA1 antibody
Host
Host: Rabbit
Source: Rat
Source: Rat
Reactivity
Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes Adenosine A1 Receptor. Species crossreactivity
Purity/Purification
Serum
Form/Format
Serum
Supplied as a liquid, neat serum, 0.1% sodium azide, 40% glycerol.
Supplied as a liquid, neat serum, 0.1% sodium azide, 40% glycerol.
Sequence Length
326
Applicable Applications for anti-ADORA1 antibody
ELISA, Western Blot
Application Notes
Suitable for use in ELISA and Western Blot. Other applications not tested.
Dilution: Western Blot: 1:1000-1:5000 using ECL. Detects 79 and 39kD bands in cortical membranes from rat, pig, and lamb. The 79kD band can be converted to 39kD band by agonist or antagonist treatment and could represent a A1R dimers. A1R, photoaffinity labeled with 125I-R-AHPIA as 39kD band.
ELISA: 1:10,000-50,000 using 50-100ng A1R control peptide/well.
Optimal dilutions to be determined by the researcher.
Dilution: Western Blot: 1:1000-1:5000 using ECL. Detects 79 and 39kD bands in cortical membranes from rat, pig, and lamb. The 79kD band can be converted to 39kD band by agonist or antagonist treatment and could represent a A1R dimers. A1R, photoaffinity labeled with 125I-R-AHPIA as 39kD band.
ELISA: 1:10,000-50,000 using 50-100ng A1R control peptide/well.
Optimal dilutions to be determined by the researcher.
Immunogen
Synthetic peptide 14aa sequence corresponding to rat A1R gene. This region corresponds to the 3rd extracellular domain, N-terminus. A cysteine has been added at the NH2 terminus for coupling to KLH.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-ADORA1 antibody
The purine nucleoside adenosine modulates diverse physiological functions including induction of sedation, vasodilatation, suppression of cardiac rate and contractility, neurotransmitter release, inhibition of platelet aggregation and lipolysis. Adenosine released from cells interacts with membrane receptors (adenosine receptors, ARs). Based upon, biochemical and pharmacological criteria, ARs have been classified into A1, A2a, A2b and A3. The high affinity receptor A1 inhibits adenyl cyclase. Low affinity receptor A2a stimulates the cyclase via G proteins. A2a receptor is believed to cause vasorelaxation in coronary artery. Various, ARs and their subtypes have recently been cloned from several species. ARs belong to the superfamily of G-protein coupled receptors. They are predicted to contain 7 transmembrane domains. The N-termini are predicted to be extracellular. The C-termini is predicted to be cytoplasmic. There is an overall 30% homology among the four ARs. ARs are distributed throughout the body. Gene location of various ARs: A1 (Chromosome 1; q.31.3-32.2); A2a (Chromosome 22); A2b (Chromosome 17; p.11.2-12); A3 (Chromos.1).
Product Categories/Family for anti-ADORA1 antibody
References
• 1. Mahan, L.C., et al., Mol. Pharmacol. 40: 1-7 (1991). 2. Reppert, S.M., et al., Mol. Endocrinol. 5: 1037-38 (1991). 3. Tucker, A.L., Linden, J., Cardiovascular Res. 27: 62-67 (1993). 4. Olah, M.E., Stile, G., Ann. Rev. Pharmacol. 35: 581-606 (1995). 5. Ciruela, F., J. Neurosci. 42: 818-828 (1995).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
36,512 Da
NCBI Official Full Name
adenosine A1 receptor
NCBI Official Synonym Full Names
adenosine A1 receptor
NCBI Official Symbol
ADORA1
NCBI Official Synonym Symbols
RDC7
NCBI Protein Information
adenosine receptor A1; OTTHUMP00000039090; OTTHUMP00000039091
UniProt Protein Name
Adenosine receptor A1
Protein Family
UniProt Gene Name
ADORA1
UniProt Entry Name
AA1R_HUMAN
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Product Notes
The ADORA1 adora1 (Catalog #AAA615163) is an Antibody produced from Host: Rabbit Source: Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti - Adenosine A1 Receptor (A1R) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Adenosine A1 Receptor can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot. Suitable for use in ELISA and Western Blot. Other applications not tested. Dilution: Western Blot: 1:1000-1:5000 using ECL. Detects 79 and 39kD bands in cortical membranes from rat, pig, and lamb. The 79kD band can be converted to 39kD band by agonist or antagonist treatment and could represent an A1R dimers. A1R, photoaffinity labeled with 125I-R-AHPIA as 39kD band. ELISA: 1:10,000-50,000 using 50-100ng A1R control peptide/well. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the ADORA1 adora1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Adenosine A1 Receptor, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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