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Western Blot (WB) (Western Blot analysis of HELA cells using Adducin a/b Polyclonal Antibody)

Rabbit Adducin alpha/beta Polyclonal Antibody | anti-ADD1 antibody

Adducin alpha/beta Polyclonal Antibody

Gene Names
ADD1; ADDA
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation, Immunofluorescence, ELISA
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
Adducin alpha/beta; Polyclonal Antibody; Adducin alpha/beta Polyclonal Antibody; Adducin a/b Polyclonal Antibody; ADD1; ADDA; Alpha-adducin; Erythrocyte adducin subunit alpha; ADD2; ADDB; Beta-adducin; Erythrocyte adducin subunit beta; anti-ADD1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
Adducin a/b Polyclonal Antibody detects endogenous levels of Adducin a/b protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration
1 mg/ml (varies by lot)
Sequence Length
737
Applicable Applications for anti-ADD1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), ELISA (EIA)
Application Notes
Western Blot: 1:500 - 1:2000
Immunohistochemistry: 1:100 - 1:300
Immunoprecipitation: 2-5 ug/mg lysate
Immunofluorescence: 1:200 - 1:1000
ELISA: 1:40000
Not yet tested in other applications.
Immunogen Type
peptide
Immunogen Description
Synthesized peptide derived from human Adducin a/b around the non-phosphorylation site of S726/713.
Target Name
Adducin a/b
Preparation and Storage
Store at -20 degree C/1 year

Western Blot (WB)

(Western Blot analysis of HELA cells using Adducin a/b Polyclonal Antibody)

Western Blot (WB) (Western Blot analysis of HELA cells using Adducin a/b Polyclonal Antibody)

Western Blot (WB)

(Western Blot analysis of HELA PC12 NIH-3T3 cells using Adducin a/b Polyclonal Antibody)

Western Blot (WB) (Western Blot analysis of HELA PC12 NIH-3T3 cells using Adducin a/b Polyclonal Antibody)
Product Categories/Family for anti-ADD1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
118
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
73,333 Da
NCBI Official Full Name
alpha-adducin isoform a
NCBI Official Synonym Full Names
adducin 1 (alpha)
NCBI Official Symbol
ADD1
NCBI Official Synonym Symbols
ADDA
NCBI Protein Information
alpha-adducin
UniProt Protein Name
Alpha-adducin
Protein Family
UniProt Gene Name
ADD1
UniProt Synonym Gene Names
ADDA
UniProt Entry Name
ADDA_HUMAN

NCBI Description

Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described. [provided by RefSeq, Jul 2008]

Uniprot Description

Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.

Research Articles on ADD1

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Product Notes

The ADD1 add1 (Catalog #AAA9401279) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Adducin alpha/beta Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Adducin alpha/beta can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), ELISA (EIA). Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:100 - 1:300 Immunoprecipitation: 2-5 ug/mg lysate Immunofluorescence: 1:200 - 1:1000 ELISA: 1:40000 Not yet tested in other applications. Researchers should empirically determine the suitability of the ADD1 add1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Adducin alpha/beta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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