Immunohistochemistry (IHC)
(ADD1 IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced retrieval.)
Rabbit ADD1 Polyclonal Antibody | anti-ADD1 antibody
ADD1, ID (ADDA, Alpha-adducin, Erythrocyte adducin subunit alpha)
Purified by immunoaffinity chromatography
Purified by immunoaffinity chromatography
Dilution: Immunofluorescence: 1:100-1:500
ELISA: 1:10,000
Immunohistochemistry (Paraffin): 10ug/ml
Immunohistochemistry (IHC)
(ADD1 IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced retrieval.)
Immunohistochemistry (IHC)
(ADD1 IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced retrieval.)
Immunofluorescence (IF)
(Immunofluorescence analysis of HeLa cells, using ADD1.The picture on the right is treated with the synthesized peptide.)
Immunofluorescence (IF)
(Immunofluorescence analysis of HeLa cells, using ADD1.The picture on the right is treated with the synthesized peptide.)
NCBI and Uniprot Product Information
NCBI Description
Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described. [provided by RefSeq, Jul 2008]
Uniprot Description
Function: Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.
Subunit structure: Heterodimer of an alpha and a beta subunit or an alpha and a gamma subunit.
Subcellular location: Cytoplasm › cytoskeleton. Cell membrane; Peripheral membrane protein; Cytoplasmic side.
Tissue specificity: Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit.
Domain: Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.
Sequence similarities: Belongs to the aldolase class II family. Adducin subfamily.
Sequence caution: The sequence CAA98970.1 differs from that shown. Reason: Erroneous gene model prediction.
Research Articles on ADD1
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Product Notes
The ADD1 add1 (Catalog #AAA649962) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ADD1, ID (ADDA, Alpha-adducin, Erythrocyte adducin subunit alpha) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ADD1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Immunohistochemistry (IHC), Immunofluorescence (IF). Suitable for use in Immunofluorescence, ELISA, Immunohistochemistry. Dilution: Immunofluorescence: 1:100-1:500 ELISA: 1:10,000 Immunohistochemistry (Paraffin): 10ug/ml. Researchers should empirically determine the suitability of the ADD1 add1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ADD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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