Immunohistochemistry (IHC)
(Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE))
Rabbit ADCY9/Adenylate Cyclase 9 Polyclonal Antibody | anti-ADCY9 antibody
Anti-ADCY9/Adenylate Cyclase 9 Antibody (N-Terminus)
Predicted: Mouse, Rat
Predicted: Mouse, Rat
IHC-P: 1:100
WB: 1:500-1:1000
Aliquot to avoid freeze/thaw cycles.
Immunohistochemistry (IHC)
(Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunohistochemistry (IHC)
(Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunofluorescence (IF)
(Immunofluorescent analysis of Adenylate Cyclase 9 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Immunofluorescence (IF)
(Immunofluorescent analysis of Adenylate Cyclase 9 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Western Blot (WB)
(Western blot analysis of Adenylate Cyclase 9 expression in HeLa (A); SP2/0 (B); H9C2 (C) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of Adenylate Cyclase 9 expression in HeLa (A); SP2/0 (B); H9C2 (C) whole cell lysates.)
NCBI and Uniprot Product Information
NCBI Description
Adenylate cyclase is a membrane bound enzyme that catalyses the formation of cyclic AMP from ATP. It is regulated by a family of G protein-coupled receptors, protein kinases, and calcium. The type 9 adenylyl cyclase is a widely distributed adenylyl cyclase, and it is stimulated by beta-adrenergic receptor activation but is insensitive to forskolin, calcium, and somatostatin. [provided by RefSeq, Jul 2008]
Uniprot Description
Adenylyl cyclase that catalyzes the formation of the signaling molecule cAMP in response to activation of G protein-coupled receptors (PubMed:9628827, PubMed:12972952, PubMed:15879435, PubMed:10987815). Contributes to signaling cascades activated by CRH (corticotropin-releasing factor), corticosteroids and beta-adrenergic receptors (PubMed:9628827).
Research Articles on ADCY9
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Product Notes
The ADCY9 adcy9 (Catalog #AAA2401476) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ADCY9/Adenylate Cyclase 9 Antibody (N-Terminus) reacts with Human Predicted: Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ADCY9/Adenylate Cyclase 9 can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). ICC: 1:100-1:500 IHC-P: 1:100 WB: 1:500-1:1000. Researchers should empirically determine the suitability of the ADCY9 adcy9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ADCY9/Adenylate Cyclase 9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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