Rabbit ADCY3 Polyclonal Antibody | anti-ADCY3 antibody

ADCY3 Antibody

Cat. Num. AAA415604

• Gene Names: ADCY3; AC3
• Applications: Immunofluorescence, Western Blot
• Purity: Affinity purified
• Synonyms: ADCY3; Polyclonal Antibody; ADCY3 Antibody; anti-ADCY3 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Purity/Purification: Affinity purified
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)
• Sequence Length: 1144

• Applicable Applications for anti-ADCY3 antibody: Immunofluorescence (IF), Western Blot (WB)
• Application Notes: The antibody can be diluted to 1:500-1,000 for immunofluorescence staining and 1:5,000-10,000 for western blotting.
• Immunogen: Adenylate cyclases are enzymes which interact with and are activated by the GTP bound alpha subunits of trimeric G-proteins. Activated adenylate cyclases are responsible for the production of the important "second messenger" signalling molecule cyclic-AMP, which is generated from ATP. The type III adenylate cyclase enzyme is localized in the membranes surrounding the cilia in neurons, and our antibody is an excellent marker of neuronal cilia in the brain and in cells in tissue culture. Adenylate cyclase type III is a large complex molecule of, in the human, 1145 amino acids with a deduced molecular weight of 129kDa. The protein may be variably glycosylated, so that on SDS-PAGE and western blots it runs as a diffuse band of about 160kDa in cortex and about 200kDa in olfactory epithelium (see below). The molecule has a complex structure, with 12 transmembrane domains and two cyclase domains. Each cyclase domain is immediately C-terminal to 6 transmembrane segments, but only the second, C-terminal cyclase is believed to be catalytically active. Our antibody was raised against a 20 amino acid peptide identical to the C-terminus of rat ACIII, which is PAAFPNGSSVTLPHQVVDNP. A cysteine residue was added to the N-terminus to allow coupling to MBS-activated keyhole limpet hemocyanin. The antibody works on human cells also, as the corresponding peptide in the human AC3 is the peptide LATFPNGPSVTLPHQVVDNS, which differs at only three amino acids. The HGNC name for this protein is ADCY3.
• Storage Buffer: PBS, pH 7.4 with 0.02% sodium azide.
• Preparation and Storage: Store at -20 degree C/1 year

Western Blot (WB)

References

1. Fuchs JL, Schwark HD. Neuronal primary cilia: a review. Cell Biol Int. 28:111-8 (2004).2. Louvi A and Grove EA. Cilia in the CNS: the quiet organelle claims center stage. Neuron 69:1046-1060 (2011).

NCBI and Uniprot Product Information

• NCBI GI #: 148536830
• NCBI GeneID: 109
• NCBI Accession #: NP_004027.2
• NCBI GenBank Nucleotide #: NM_004036.3
• UniProt Accession #: O60266; Q53T54; Q9UDB1
• Molecular Weight: 128,960 Da
• NCBI Official Full Name: adenylate cyclase type 3
• NCBI Official Synonym Full Names: adenylate cyclase 3
• NCBI Official Symbol: ADCY3
• NCBI Official Synonym Symbols: AC3
• NCBI Protein Information: adenylate cyclase type 3; AC-III; adenylyl cyclase 3; ATP pyrophosphate-lyase 3; adenylate cyclase type III; adenylyl cyclase, type III; adenylate cyclase, olfactive type
• UniProt Protein Name: Adenylate cyclase type 3
• Protein Family: Adenylate cyclase
• UniProt Gene Name: ADCY3
• UniProt Synonym Gene Names: KIAA0511; AC-III; AC3
• UniProt Entry Name: ADCY3_HUMAN

NCBI Description

This gene encodes adenylyl cyclase 3 which is a membrane-associated enzyme and catalyzes the formation of the secondary messenger cyclic adenosine monophosphate (cAMP). This protein appears to be widely expressed in various human tissues and may be involved in a number of physiological and pathophysiological metabolic processes. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Mediates odorant detection (possibly) via modulation of intracellular cAMP concentration.

Catalytic activity: ATP = 3',5'-cyclic AMP + diphosphate.

Cofactor: Binds 2 magnesium ions per subunit

By similarity.

Enzyme regulation: Activated by calcium/calmodulin.

Subcellular location: Membrane; Multi-pass membrane protein.

Tissue specificity: Expressed in brain, heart, kidney, liver, lung, pancreas, placenta, and skeletal muscle.

Sequence similarities: Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.Contains 2 guanylate cyclase domains.