Rabbit ADAR2 Polyclonal Antibody | anti-ADAR2 antibody

ADAR2 (R510) Polyclonal Antibody

Cat. Num. AAA3002068

• Gene Names: ADARB1; RED1; ADAR2; DRABA2; DRADA2; NEDHYMS
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ADAR2; Polyclonal Antibody; ADAR2 (R510) Polyclonal Antibody; ADARB1; RNA-editing deaminase 1; DRADA2; RED1; dsRNA adenosine deaminase; Double-stranded RNA-specific editase 1; anti-ADAR2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ADAR2 (R510) polyclonal antibody detects endogenous levels of ADAR2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 701

• Applicable Applications for anti-ADAR2 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 480-530 of Human ADAR2.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of ADAR2 (R510) pAb at 1:1000 dilutionLane1:L02 whole cell lysate (20ug) Lane2:H9C2 whole cell lysate (40ug) Lane3:CT26 whole cell lysate (40ug) Lane4:MEF whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of ADAR2 (R510) pAb in paraffin-embedded human colon carcinoma tissue at 1:50,showing cytoplasm and nuclear staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of ADAR2 (R510) pAb in paraffin-embedded human lung carcinoma tissue at 1:50,showing cytoplasm and nuclear staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Related Product Information for anti-ADAR2 antibody

ADAR2, also designated adenosine deaminase, RNA-specific (RED1), RNA-editing enzyme 1, DRABA2, DRADA2, ADAR2alpha-L1, ADAR2alpha-L2 and ADAR2alpha-L3, mediates RNA editing by destabilizing RNA through deamination of adenosine to inosine. ADAR2 is responsible for pre-mRNA editing of the glutamate receptor subunit B by site-specific deamination of adenosines. It can modify its own pre-mRNA and generate new splice sites. Translocation of endogenous ADAR2 from the nucleolus to the nucleoplasm results in increased editing of endogenous ADAR2 substrates. Alternative splicing of this gene results in several transcript variants that may influence RNA editing. RNA editing involves the deamination of adenosines at specific sites, the result of which can be a change in the amino acid sequence of the protein so that it differs from that predicted by the sequence of the DNA.

NCBI and Uniprot Product Information

• NCBI GI #: 4501919
• NCBI GeneID: 104
• NCBI Accession #: NP_001103.1
• NCBI GenBank Nucleotide #: NP_001103.1
• UniProt Accession #: P78563
• Molecular Weight: ~ 82kDa
• NCBI Official Full Name: double-stranded RNA-specific editase 1 isoform 1
• NCBI Official Synonym Full Names: adenosine deaminase RNA specific B1
• NCBI Official Symbol: ADARB1
• NCBI Official Synonym Symbols: RED1; ADAR2; DRABA2; DRADA2; NEDHYMS
• NCBI Protein Information: double-stranded RNA-specific editase 1
• UniProt Protein Name: Double-stranded RNA-specific editase 1
• UniProt Gene Name: ADARB1
• UniProt Synonym Gene Names: ADAR2; DRADA2; RED1
• UniProt Entry Name: RED1_HUMAN

NCBI Description

This gene encodes the enzyme responsible for pre-mRNA editing of the glutamate receptor subunit B by site-specific deamination of adenosines. Studies in rat found that this enzyme acted on its own pre-mRNA molecules to convert an AA dinucleotide to an AI dinucleotide which resulted in a new splice site. Alternative splicing of this gene results in several transcript variants, some of which have been characterized by the presence or absence of an ALU cassette insert and a short or long C-terminal region. [provided by RefSeq, Jul 2008]

Uniprot Description

RED1: Editing of the messenger RNAs for glutamate receptor (GluR) subunits by site-selective adenosine deamination. Edits both the GluR-B Q/R and R/G sites efficiently but converts the adenosine in hotspot1 much less efficiently. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Nucleolus; Hydrolase; EC 3.5.4.37

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: nucleoplasm; cytoplasm; nucleolus; nucleus

Molecular Function: adenosine deaminase activity; mRNA binding; double-stranded RNA adenosine deaminase activity; protein binding; RNA binding; metal ion binding; double-stranded RNA binding

Biological Process: RNA processing; negative regulation of cell proliferation; base conversion or substitution editing; positive regulation of viral genome replication; regulation of cell cycle; mRNA modification; innate immune response; gene expression; mRNA processing; defense response to virus; adenosine to inosine editing; negative regulation of cell migration