Western Blot (WB)
(Western blot analysis of ADAMTS9 using Jurkat whole cell lysates)
Rabbit anti-Human ADAMTS9 Polyclonal Antibody | anti-ADAMTS9 antibody
ADAMTS9 Antibody
Reactivity
Human
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
ADAMTS9; Polyclonal Antibody; ADAMTS9 Antibody; A disintegrin and metalloproteinase with thrombospondin motifs 9; A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 9; A disintegrin like and metalloprotease with thrombospondin type 1 motif 9; ADAM metallopeptidase with thrombospondin type 1 motif 9; ADAM TS 9; ADAM TS9; ADAMTS 9; FLJ42955; KIAA1312; anti-ADAMTS9 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
ADAMTS9 antibody detects endogenous levels of total ADAMTS9
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
1907
Applicable Applications for anti-ADAMTS9 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000-3000
Immunogen
A synthesized peptide derived from human ADAMTS9
Subcellular Location
Secreted Protein; Extracellular Space; Extracellular Matrix.
Tissue Specificity
Highly expressed in all fetal tissues. Expressed in a number of adult tissues with highest expression in heart, placenta and skeletal muscle.
Predicted Cross Reactivity
Pig, Horse, Dog
Similarity
Pig (80%), Horse (90%), Dog (86%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of ADAMTS9 using Jurkat whole cell lysates)
Western Blot (WB)
(Western blot analysis of ADAMTS9 using Jurkat whole cell lysates)
Related Product Information for anti-ADAMTS9 antibody
Function: Cleaves the large aggregating proteoglycans, aggrecan (at the '1838-Glu-|-Ala-1839' site) and versican (at the '1428-Glu-|-Ala-1429' site). Has a protease-independent function in promoting the transport from the endoplasmic reticulum to the Golgi apparatus of a variety of secretory cargos.
Post-translational Modifications: The precursor is cleaved by a furin endopeptidase. N-glycosylated (PubMed:12514189). Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X2-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1, 3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion (By similarity).
Similarity: The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.The ancillary domains, including the TSRs domain, are required for specific extracellular localization and for its versicanase and aggrecanase activities.The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.The GON domain mediates protease-independent function in ER to Golgi transport.
Post-translational Modifications: The precursor is cleaved by a furin endopeptidase. N-glycosylated (PubMed:12514189). Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X2-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1, 3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion (By similarity).
Similarity: The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.The ancillary domains, including the TSRs domain, are required for specific extracellular localization and for its versicanase and aggrecanase activities.The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.The GON domain mediates protease-independent function in ER to Golgi transport.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 216 kDa
Predicted: 217 kDa
Predicted: 217 kDa
NCBI Official Full Name
A disintegrin and metalloproteinase with thrombospondin motifs 9 isoform 4
NCBI Official Synonym Full Names
ADAM metallopeptidase with thrombospondin type 1 motif 9
NCBI Official Symbol
ADAMTS9
NCBI Protein Information
A disintegrin and metalloproteinase with thrombospondin motifs 9
UniProt Protein Name
A disintegrin and metalloproteinase with thrombospondin motifs 9
UniProt Gene Name
ADAMTS9
UniProt Synonym Gene Names
KIAA1312; ADAM-TS 9; ADAM-TS9; ADAMTS-9
NCBI Description
This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. Members of the ADAMTS family have been implicated in the cleavage of proteoglycans, the control of organ shape during development, and the inhibition of angiogenesis. This gene is localized to chromosome 3p14.3-p14.2, an area known to be lost in hereditary renal tumors. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing. [provided by RefSeq, Jan 2016]
Uniprot Description
Cleaves the large aggregating proteoglycans, aggrecan (at the '1838-Glu-|-Ala-1839' site) and versican (at the '1428-Glu-|-Ala-1429' site). Has a protease-independent function in promoting the transport from the endoplasmic reticulum to the Golgi apparatus of a variety of secretory cargos.
Research Articles on ADAMTS9
Similar Products
Product Notes
The ADAMTS9 adamts9 (Catalog #AAA9609495) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ADAMTS9 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ADAMTS9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000-3000. Researchers should empirically determine the suitability of the ADAMTS9 adamts9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ADAMTS9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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