Rabbit anti-Human, Mouse ADAMTS15 Polyclonal Antibody | anti-ADAMTS15 antibody

ADAMTS15 Antibody

Cat. Num. AAA9629069

• Reactivity: Human, Mouse
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: ADAMTS15; Polyclonal Antibody; ADAMTS15 Antibody; A disintegrin and metalloproteinase with thrombospondin motifs 15; A disintegrin like and metalloprotease reprolysin type with thrombospondin type 1 motif 15; A disintegrin like and metalloprotease reprolysin type with thrombospondin type 1 motif 15 preproprotein; A disintegrin like and metalloprotease with thrombospondin type 1 motif 15; A disintegrin like and metalloprotease with thrombospondin type 1 motif 15 preproprotein; ADAM metallopeptidase with thrombospondin type 1 motif 15; ADAM TS 15; ADAM TS15; ADAM-TS 15; ADAM-TS15; ADAMTS 15; ADAMTS-15; ATS15_HUMAN; MGC126403; anti-ADAMTS15 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: ADAMTS15 Antibody detects endogenous levels of total ADAMTS15.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-ADAMTS15 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from Human MYOCD.
• Conjugation: Unconjugated
• Similarity: The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix. The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
• Post Translational Modifications: The precursor is cleaved by a furin endopeptidase.Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1, 3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion (By similarity).
• Tissue Specificity: Expressed in fetal liver and kidney, but not in any of the adult tissues examined.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-ADAMTS15 antibody

Secreted; Extracellular space; Extracellular matrix; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 21265058
• NCBI GeneID: 170689
• NCBI Accession #: NP_620686.1
• NCBI GenBank Nucleotide #: NM_139055.2
• UniProt Accession #: Q8TE58; Q32MI6
• Molecular Weight: 103,287 Da
• NCBI Official Full Name: A disintegrin and metalloproteinase with thrombospondin motifs 15 preproprotein
• NCBI Official Synonym Full Names: ADAM metallopeptidase with thrombospondin type 1 motif, 15
• NCBI Official Symbol: ADAMTS15
• NCBI Protein Information: A disintegrin and metalloproteinase with thrombospondin motifs 15; ADAM-TS15; ADAMTS-15; ADAM-TS 15; metalloprotease disintegrin 15 with thrombospondin domains; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 15,
• UniProt Protein Name: A disintegrin and metalloproteinase with thrombospondin motifs 15
• Protein Family: A disintegrin and metalloproteinase with thrombospondin motifs
• UniProt Gene Name: ADAMTS15
• UniProt Synonym Gene Names: ADAM-TS 15; ADAM-TS15; ADAMTS-15
• UniProt Entry Name: ATS15_HUMAN

NCBI Description

This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. ADAMTS family members share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The protein encoded by this gene has a high sequence similarity to the proteins encoded by ADAMTS1 and ADAMTS8. [provided by RefSeq, Jul 2008]

Uniprot Description

ADAMTS15:

Protein type: Protease; Motility/polarity/chemotaxis; Extracellular matrix; EC 3.4.24.-; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11q25

Cellular Component: proteinaceous extracellular matrix

Molecular Function: zinc ion binding; metalloendopeptidase activity

Biological Process: proteolysis