Rabbit anti-Human, Mouse ADAM9 Polyclonal Antibody | anti-ADAM9 antibody

ADAM9, NT (ADAM9, KIAA0021, MCMP, MDC9, MLTNG, Disintegrin and metalloproteinase domain-containing protein 9, Cellular disintegrin-related protein, Meltrin-gamma, Metalloprotease/disintegrin/cysteine-rich protein 9, Myeloma cell metalloproteinase) (HRP)

Cat. Num. AAA6271567

• Gene Names: ADAM9; MCMP; MDC9; CORD9; Mltng
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, ELISA, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: ADAM9; Polyclonal Antibody; NT (ADAM9; KIAA0021; MCMP; MDC9; MLTNG; Disintegrin and metalloproteinase domain-containing protein 9; Cellular disintegrin-related protein; Meltrin-gamma; Metalloprotease/disintegrin/cysteine-rich protein 9; Myeloma cell metalloproteinase) (HRP); Myeloma cell metalloproteinase; anti-ADAM9 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-ADAM9 antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: ADAM9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 42~72 amino acids from the N-terminal region of human ADAM9.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Western Blot (WB)

(Western blot analysis of anti-ADAM9 Antibody (N-term) in mouse kidney tissue lysates (35ug/lane). ADAM9 (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of HepG2 cells using ADAM9 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-ADAM9 antibody

Antibodies; Matrix Metalloproteinases

References

Weskamp G., Kraetzschmar J., Reid M.S.J. Cell Biol. 132:717-726(1996). Hotoda N., Koike H.Biochem. Biophys. Res. Commun. 293:800-805(2002). McKie N., Edwards T., Dallas D.J.Biochem. Biophys. Res. Commun. 230:335-339(1997).

NCBI and Uniprot Product Information

• NCBI GI #: 116496893
• NCBI GeneID: 8754
• UniProt Accession #: Q13443; Q10718; Q8NFM6; B7ZLN7
• Molecular Weight: 90,556 Da
• NCBI Official Full Name: ADAM9 protein
• NCBI Official Synonym Full Names: ADAM metallopeptidase domain 9
• NCBI Official Symbol: ADAM9
• NCBI Official Synonym Symbols: MCMP; MDC9; CORD9; Mltng
• NCBI Protein Information: disintegrin and metalloproteinase domain-containing protein 9; cone rod dystrophy 9; myeloma cell metalloproteinase; cellular disintegrin-related protein; ADAM metallopeptidase domain 9 (meltrin gamma); metalloprotease/disintegrin/cysteine-rich protein 9
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 9
• Protein Family: Disintegrin and metalloproteinase domain-containing protein
• UniProt Gene Name: ADAM9
• UniProt Synonym Gene Names: KIAA0021; MCMP; MDC9; MLTNG; ADAM 9
• UniProt Entry Name: ADAM9_HUMAN

NCBI Description

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene interacts with SH3 domain-containing proteins, binds mitotic arrest deficient 2 beta protein, and is also involved in TPA-induced ectodomain shedding of membrane-anchored heparin-binding EGF-like growth factor. Several alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Jul 2010]

Uniprot Description

ADAM9: Probable zinc protease. May mediate cell-cell or cell- matrix interactions. Isoform 2 displays alpha-secretase activity for APP. Widely expressed. Expressed in chondrocytes. Isoform 2 is highly expressed in liver and heart. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Protease; Motility/polarity/chemotaxis; EC 3.4.24.-

Chromosomal Location of Human Ortholog: 8p11.22

Cellular Component: extracellular space; focal adhesion; cell surface; basolateral plasma membrane; cytoplasm; integral to membrane

Molecular Function: collagen binding; integrin binding; protein binding; protein kinase C binding; zinc ion binding; metallopeptidase activity; metalloendopeptidase activity; laminin binding; SH3 domain binding

Biological Process: integrin-mediated signaling pathway; positive regulation of keratinocyte migration; extracellular matrix organization and biogenesis; activation of MAPKK activity; cell-matrix adhesion; membrane protein ectodomain proteolysis; response to glucocorticoid stimulus; positive regulation of cell adhesion mediated by integrin; positive regulation of membrane protein ectodomain proteolysis; keratinocyte differentiation; response to manganese ion; collagen catabolic process; extracellular matrix disassembly; PMA-inducible membrane protein ectodomain proteolysis; monocyte activation; response to hydrogen peroxide; positive regulation of protein secretion; transforming growth factor beta receptor signaling pathway; cell-cell adhesion mediated by integrin; cell adhesion mediated by integrin; cell adhesion; response to calcium ion

Disease: Cone-rod Dystrophy 9