Rabbit anti-Human HSD17B3 Polyclonal Antibody | anti-HSD17B3 antibody

HSD17B3, ID (HSD17B3, EDH17B3, Testosterone 17-beta-dehydrogenase 3, 17-beta-hydroxysteroid dehydrogenase type 3, Testicular 17-beta-hydroxysteroid dehydrogenase) (Azide free) (HRP)

Cat. Num. AAA6308500

• Gene Names: HSD17B3; EDH17B3; SDR12C2
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: HSD17B3; Polyclonal Antibody; ID (HSD17B3; EDH17B3; Testosterone 17-beta-dehydrogenase 3; 17-beta-hydroxysteroid dehydrogenase type 3; Testicular 17-beta-hydroxysteroid dehydrogenase) (Azide free) (HRP); Testicular 17-beta-hydroxysteroid dehydrogenase; anti-HSD17B3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HSD17B3.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-HSD17B3 antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 89-118 in the central region of human HSD17B3, UniProt Accession #P37058.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of K562 cell line lysates (35ug/lane) usingMBS6012152. HSD17B3 (arrow) was detected usingMBS6012152.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human testis tissue usingMBS6012152, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS6012152 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometry analysis of K562 cells (bottom histogram) using MBS6012152 compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-HSD17B3 antibody

This isoform of 17 beta-hydroxysteroid dehydrogenase is expressed predominantly in the testis and catalyzes the conversion of androstenedione to testosterone. It preferentially uses NADP as cofactor. Deficiency can result in male pseudohermaphroditism with gynecomastia.

Product Categories/Family for anti-HSD17B3 antibody

Antibodies; Enzymes; Dehydrogenase

References

1. Li, J., et al. Breast Cancer Res. 12 (2), R19 (2010).2. Sata, F., et al. J Sex Med (2010). 3. Ahn, J., et al. Hum. Mol. Genet. 18(19):3749-3757(2009). 4. Chakrabarti, B., et al. Autism Res 2(3):157-177(2009). 5. Beuten, J., et al. Cancer Epidemiol. Biomarkers Prev. 18(6):1869-1880(2009). 6. Andersson, S., et al. J. Clin. Endocrinol. Metab. 81(1):130-136(1996).

NCBI and Uniprot Product Information

• NCBI GI #: 4557649
• NCBI GeneID: 3293
• NCBI Accession #: NP_000188.1
• NCBI GenBank Nucleotide #: NM_000197.1
• UniProt Accession #: P37058
• Molecular Weight: 34,516 Da
• NCBI Official Full Name: testosterone 17-beta-dehydrogenase 3
• NCBI Official Synonym Full Names: hydroxysteroid (17-beta) dehydrogenase 3
• NCBI Official Symbol: HSD17B3
• NCBI Official Synonym Symbols: EDH17B3; SDR12C2
• NCBI Protein Information: testosterone 17-beta-dehydrogenase 3; 17-beta-HSD3; 17-beta-HSD 3; 17-beta-hydroxysteroid dehydrogenase type 3; testicular 17-beta-hydroxysteroid dehydrogenase; short chain dehydrogenase/reductase family 12C, member 2
• UniProt Protein Name: Testosterone 17-beta-dehydrogenase 3
• Protein Family: Testosterone 17-beta-dehydrogenase
• UniProt Gene Name: HSD17B3
• UniProt Synonym Gene Names: EDH17B3; 17-beta-HSD 3
• UniProt Entry Name: DHB3_HUMAN

NCBI Description

This isoform of 17 beta-hydroxysteroid dehydrogenase is expressed predominantly in the testis and catalyzes the conversion of androstenedione to testosterone. It preferentially uses NADP as cofactor. Deficiency can result in male pseudohermaphroditism with gynecomastia. [provided by RefSeq, Jul 2008]

Uniprot Description

HSD17B3: Favors the reduction of androstenedione to testosterone. Uses NADPH while the two other EDH17B enzymes use NADH. Defects in HSD17B3 are the cause of male pseudohermaphrodism with gynecomastia (MPH). These individuals have unambiguous female external genitalia at birth, but fail to menstruate at the time of expected puberty and instead virilize as evidenced by growth of the phallus. Breast development may or may not take place. Belongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily.

Protein type: EC 1.1.1.64; Oxidoreductase; Lipid Metabolism - androgen and estrogen

Chromosomal Location of Human Ortholog: 9q22

Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle

Molecular Function: testosterone 17-beta-dehydrogenase (NADP+) activity

Biological Process: steroid metabolic process; male genitalia development; androgen biosynthetic process

Disease: 17-beta Hydroxysteroid Dehydrogenase Iii Deficiency