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Western Blot (WB) (Western blot analysis of ADAM8 using HepG2 whole cell lysates)

Rabbit anti-Human, Rat ADAM8 Polyclonal Antibody | anti-ADAM8 antibody

ADAM8 Antibody

Gene Names
ADAM8; MS2; CD156; CD156a
Reactivity
Human, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
ADAM8; Polyclonal Antibody; ADAM8 Antibody; A Disintegrin And Metalloproteinase domain 8; ADAM 8; ADAM 8 precursor; ADAM metallopeptidase domain 8; Adam8; ADAM8 protein; ADAM8_HUMAN; CD 156; CD156; CD156a; CD156a antigen; Cell surface antigen MS2; Disintegrin and metalloproteinase domain-containing protein 8; Human leukocyte differentiation antigen; Macrophage cysteine rich glycoprotein; MGC134985; MS 2; MS2; anti-ADAM8 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
ADAM8 antibody detects endogenous levels of total ADAM8
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
824
Applicable Applications for anti-ADAM8 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:1000-3000
IHC: 1:200
Immunogen
A synthesized peptide derived from human ADAM8
Subcellular Location
Membrane.
Tissue Specificity
Expressed on neutrophils and monocytes.
Predicted Cross Reactivity
Pig, Bovine, Dog
Similarity
Pig (89%), Bovine (89%), Dog (89%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of ADAM8 using HepG2 whole cell lysates)

Western Blot (WB) (Western blot analysis of ADAM8 using HepG2 whole cell lysates)

Immunohistochemistry (IHC)

(MBS9603011 at 1/100 staining Rat colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Immunohistochemistry (IHC) (MBS9603011 at 1/100 staining Rat colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-ADAM8 antibody
Function: Possible involvement in extravasation of leukocytes.
Subunit Structure: Interacts with FST3.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
101
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 89 kDa
Predicted: 89 kDa
NCBI Official Full Name
disintegrin and metalloproteinase domain-containing protein 8 isoform 1
NCBI Official Synonym Full Names
ADAM metallopeptidase domain 8
NCBI Official Symbol
ADAM8
NCBI Official Synonym Symbols
MS2; CD156; CD156a
NCBI Protein Information
disintegrin and metalloproteinase domain-containing protein 8
UniProt Protein Name
Disintegrin and metalloproteinase domain-containing protein 8
UniProt Gene Name
ADAM8
UniProt Synonym Gene Names
MS2; ADAM 8

NCBI Description

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene may be involved in cell adhesion during neurodegeneration, and it is thought to be a target for allergic respiratory diseases, including asthma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2009]

Uniprot Description

Possible involvement in extravasation of leukocytes.

Research Articles on ADAM8

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Product Notes

The ADAM8 adam8 (Catalog #AAA9603011) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ADAM8 Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ADAM8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:1000-3000 IHC: 1:200. Researchers should empirically determine the suitability of the ADAM8 adam8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ADAM8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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