Rabbit anti-Human ADAM19 Polyclonal Antibody | anti-ADAM19 antibody

ADAM19, ID (ADAM19, MLTNB, Disintegrin and metalloproteinase domain-containing protein 19, Meltrin-beta, Metalloprotease and disintegrin dendritic antigen marker) (MaxLight 405)

Cat. Num. AAA6271513

• Gene Names: ADAM19; MLTNB; FKSG34; MADDAM
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: ADAM19; Polyclonal Antibody; ID (ADAM19; MLTNB; Disintegrin and metalloproteinase domain-containing protein 19; Meltrin-beta; Metalloprotease and disintegrin dendritic antigen marker) (MaxLight 405); Metalloprotease and disintegrin dendritic antigen marker; anti-ADAM19 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ADAM19
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-ADAM19 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: ADAM19 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 215~245 amino acids from the Central region of human ADAM19.
• Conjugate: MaxLight405
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ADAM19 Antibody (Center) in WiDr cell line lysates (35ug/lane). ADAM19 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(ADAM19 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ADAM19 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(ADAM19 Antibody (Center) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-ADAM19 antibody

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This member is a type I transmembrane protein and serves as a marker for dendritic cell differentiation. It has also been demonstrated to be an active metalloproteinase, which may be involved in normal physiological and pathological processes such as cells migration, cell adhesion, cell-cell and cell-matrix interactions, and signal transduction.

Product Categories/Family for anti-ADAM19 antibody

Antibodies; Matrix Metalloproteinases

References

Hancock, D.B., et al. Nat. Genet. 42(1):45-52(2010). Styrkarsdottir, U., et al. Nat. Genet. 41(1):15-17(2009). Chan, M.W., et al. Neoplasia 10(9):908-919(2008). Tanabe, C., et al. Biochem. Biophys. Res. Commun. 352(1):111-117(2007). Melenhorst, W.B., et al. Am. J. Transplant. 6(7):1673-1681(2006). Kang, T., et al. J. Biol. Chem. 277(28):25583-25591(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 38197238
• NCBI GeneID: 8728
• UniProt Accession #: Q9H013; Q9BZL5; Q9UHP2
• Molecular Weight: 104,997 Da
• NCBI Official Full Name: ADAM19 protein, partial
• NCBI Official Synonym Full Names: ADAM metallopeptidase domain 19
• NCBI Official Symbol: ADAM19
• NCBI Official Synonym Symbols: MLTNB; FKSG34; MADDAM
• NCBI Protein Information: disintegrin and metalloproteinase domain-containing protein 19; ADAM 19; meltrin-beta; metalloprotease-disintegrin meltrin beta; metalloprotease and disintegrin dendritic antigen marker; a disintegrin and metalloproteinase domain 19 (meltrin beta)
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 19
• Protein Family: Disintegrin and metalloproteinase domain-containing protein
• UniProt Gene Name: ADAM19
• UniProt Synonym Gene Names: MLTNB; ADAM 19; MADDAM
• UniProt Entry Name: ADA19_HUMAN

NCBI Description

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This member is a type I transmembrane protein and serves as a marker for dendritic cell differentiation. It has been demonstrated to be an active metalloproteinase, which may be involved in normal physiological processes such as cell migration, cell adhesion, cell-cell and cell-matrix interactions, and signal transduction. It is proposed to play a role in pathological processes, such as cancer, inflammatory diseases, renal diseases, and Alzheimer's disease. [provided by RefSeq, May 2013]

Uniprot Description

ADAM19: Participates in the proteolytic processing of beta-type neuregulin isoforms which are involved in neurogenesis and synaptogenesis, suggesting a regulatory role in glial cell. Also cleaves alpha-2 macroglobulin. May be involved in osteoblast differentiation and/or osteoblast activity in bone. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Protease; EC 3.4.24.-

Chromosomal Location of Human Ortholog: 5q33.3

Cellular Component: integral to membrane

Molecular Function: zinc ion binding; metalloendopeptidase activity; SH3 domain binding

Biological Process: heart development; membrane protein ectodomain proteolysis