Rabbit anti-Human ADAM10/KUZ Polyclonal Antibody | anti-ADAM10/KUZ antibody

Rabbit anti ADAM10/KUZ Polyclonal Antibody

Cat. Num. AAA460301

• Gene Names: ADAM10; RAK; kuz; AD10; AD18; MADM; CD156c; CDw156; HsT18717
• Reactivity: Human
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by Epitope Affinity Purification
• Synonyms: ADAM10/KUZ; Polyclonal Antibody; Rabbit anti ADAM10/KUZ Polyclonal Antibody; ADAM metallopeptidase domain 10; kuz; AD10; MADM; CD156c; anti-ADAM10/KUZ antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This antibody recognizes ~82 kDa of ADAM10 protein from human, rat and mouse origins. The other species are not tested.
• Purity/Purification: Purified by Epitope Affinity Purification
• Form/Format: Supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
• Sequence Length: 512

• Applicable Applications for anti-ADAM10/KUZ antibody: ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: ELISA: 0.01-0.1 ug/ml; Flow Cytometry: 5-10 ug/ml; Immunohistochemistry: 2-10 ug/ml; Immunoprecipitation: 2-5 ug/ml; Western Blot: 0.1-1 ug/ml
• Preparation: A synthetic peptide corresponding to the C-terminus of ADAM10 protein from human, mouse and rat origins.
• Preparation and Storage: The antibodies are stable for 12 months from date of receipt when stored at -20 degree C. The antibodies can be stored at 2 degree C - 8 degree C for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Related Product Information for anti-ADAM10/KUZ antibody

ADAM10 is a member of the ADAM (A Disintegrin And Metalloprotease) family of membrane-anchored glycoproteins comprised of several distinct protein modules, including a pro- and metalloprotease domain, disintegrin domain, cysteine-rich region and an EGF repeat. These proteases perform essential functions in cell adhesion and fusion in diverse systems and participate in ectodomain shedding of molecules such as TNF-a and Drosophila signaling molecule Notch.

References

J. Fujita, T. Takeuchi, N. Dobashi, Y. Ohtsuki, M. Tokuda, and J. Takahara; Detection of anti-ADAM 10 antibody in serum of a patient with pulmonary fibrosis associated with dermatomyositis; Ann Rheum Dis. 1999 December; 58 (12): 770 -772.

NCBI and Uniprot Product Information

• NCBI GI #: 116496847
• NCBI GeneID: 102
• Molecular Weight: 49,047 Da
• NCBI Official Full Name: ADAM10 protein
• NCBI Official Synonym Full Names: ADAM metallopeptidase domain 10
• NCBI Official Symbol: ADAM10
• NCBI Official Synonym Symbols: RAK; kuz; AD10; AD18; MADM; CD156c; CDw156; HsT18717
• NCBI Protein Information: disintegrin and metalloproteinase domain-containing protein 10
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 10
• UniProt Gene Name: ADAM10
• UniProt Synonym Gene Names: KUZ; MADM; ADAM 10

NCBI Description

Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. Alternate splicing results in multiple transcript variants encoding different proteins that may undergo similar processing. [provided by RefSeq, Feb 2016]

Uniprot Description

ADAM10: Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-|-Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth- like factor, ephrin-A2 and for constitutive and regulated alpha- secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis. Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form. Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B. May regulate the EFNA5-EPHA3 signaling.

Protein type: Cell adhesion; Cell surface; EC 3.4.24.81; Membrane protein, integral; Motility/polarity/chemotaxis; Protease; Vesicle

Chromosomal Location of Human Ortholog: 15q21.3

Cellular Component: cell surface; cytoplasm; endoplasmic reticulum lumen; focal adhesion; Golgi apparatus; Golgi-associated vesicle; membrane; nucleus; plasma membrane

Molecular Function: endopeptidase activity; metalloendopeptidase activity; metallopeptidase activity; protein binding; protein homodimerization activity; protein kinase binding

Biological Process: cellular protein metabolic process; constitutive protein ectodomain proteolysis; ephrin receptor signaling pathway; extracellular matrix disassembly; in utero embryonic development; membrane protein ectodomain proteolysis; monocyte activation; negative regulation of cell adhesion; neutrophil degranulation; Notch receptor processing; Notch signaling pathway; PMA-inducible membrane protein ectodomain proteolysis; positive regulation of cell growth; positive regulation of cell migration; positive regulation of cell proliferation; post-translational protein modification; protein amino acid phosphorylation

Disease: Alzheimer Disease 18; Reticulate Acropigmentation Of Kitamura