Rabbit ACTR-IIA Polyclonal Antibody | anti-ACTR-IIA antibody

ACTR-IIA (H59) Polyclonal Antibody

Cat. Num. AAA3004112

• Gene Names: ACVR2A; ACVR2; ACTRII
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ACTR-IIA; Polyclonal Antibody; ACTR-IIA (H59) Polyclonal Antibody; Activin receptor type-2A; EC=2.7.11.30; Activin receptor type IIA; ACTRIIA; ACVR2A; ACVR2; anti-ACTR-IIA antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ACTR-IIA (H59) polyclonal antibody detects endogenous levels of ACTR-IIA protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 513

• Applicable Applications for anti-ACTR-IIA antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 30-80 of Human ACTR-IIA.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of ACTR-IIA (H59) pAb at 1:500 dilutionLane1:AML-12 whole cell lysate (40ug) Lane2:C6 whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:A549 whole cell lysate (40ug))

Related Product Information for anti-ACTR-IIA antibody

Members of the transforming growth factor beta superfamily bind to a pair of transmembrane proteins, known as receptor types I and II, which contain serine/threonine kinases and associate to form a signaling complex. Activin has been shown to bind a heteromeric noncovalent complex, which consists of a type I receptor, ACTR-IA (also designated ACVRI and ALK-2) or ACTR-IB (also designated ALK-4 and SKR2), and a type II receptor, ACTR-IIA (ACVR2A) or ACTR-IIB (ACVR2B). Both receptor types are highly expressed in brain. The activin receptor family members are thought to mediate distinct effects on gene expression, cell differentiation and morphogenesis in a dose dependent manner.

NCBI and Uniprot Product Information

• NCBI GI #: 178050
• NCBI GeneID: 92
• UniProt Accession #: P27037
• Molecular Weight: ~ 48kDa
• NCBI Official Full Name: activin type II receptor
• NCBI Official Synonym Full Names: activin A receptor type 2A
• NCBI Official Symbol: ACVR2A
• NCBI Official Synonym Symbols: ACVR2; ACTRII
• NCBI Protein Information: activin receptor type-2A
• UniProt Protein Name: Activin receptor type-2A
• UniProt Gene Name: ACVR2A
• UniProt Synonym Gene Names: ACVR2; ACTR-IIA; ACTRIIA
• UniProt Entry Name: AVR2A_HUMAN

NCBI Description

This gene encodes a receptor that mediates the functions of activins, which are members of the transforming growth factor-beta (TGF-beta) superfamily involved in diverse biological processes. The encoded protein is a transmembrane serine-threonine kinase receptor which mediates signaling by forming heterodimeric complexes with various combinations of type I and type II receptors and ligands in a cell-specific manner. The encoded type II receptor is primarily involved in ligand-binding and includes an extracellular ligand-binding domain, a transmembrane domain and a cytoplasmic serine-threonine kinase domain. This gene may be associated with susceptibility to preeclampsia, a pregnancy-related disease which can result in maternal and fetal morbidity and mortality. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jun 2013]

Uniprot Description

ACVR2A: a tyrosine-kinase like receptor kinase of the STKR family. The holoreceptor receptor is a heteromeric complex of receptor serine kinases which include at least two type I (I and IB) and two type II (II and IIB) receptors. Each is composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic kinase domain. Type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. Type II receptor kinases are apparently constitutively active.

Protein type: EC 2.7.11.30; Protein kinase, TKL; Kinase, protein; Motility/polarity/chemotaxis; Membrane protein, integral; Protein kinase, Ser/Thr (receptor); TKL group; STKR family; Type2 subfamily

Chromosomal Location of Human Ortholog: 2q22.3

Cellular Component: cell surface; integral to plasma membrane; cytoplasm; plasma membrane; receptor complex

Molecular Function: activin receptor activity; protein self-association; metal ion binding; transmembrane receptor protein serine/threonine kinase activity; PDZ domain binding; transforming growth factor beta receptor activity; protein serine/threonine kinase activity; protein binding; growth factor binding; activin binding; coreceptor activity; ATP binding; receptor signaling protein serine/threonine kinase activity

Biological Process: positive regulation of erythrocyte differentiation; embryonic skeletal development; regulation of BMP signaling pathway; activin receptor signaling pathway; Sertoli cell proliferation; gastrulation with mouth forming second; positive regulation of bone mineralization; protein amino acid phosphorylation; BMP signaling pathway; anterior/posterior pattern formation; positive regulation of osteoblast differentiation; positive regulation of activin receptor signaling pathway; transmembrane receptor protein serine/threonine kinase signaling pathway; mesoderm development; spermatogenesis; regulation of nitric-oxide synthase activity; sperm ejaculation; positive regulation of protein amino acid phosphorylation; determination of left/right symmetry; penile erection