Rabbit anti-Human ACTN3 Polyclonal Antibody | anti-ACTN3 antibody

ACTN3, ID (ACTN3, Alpha-actinin-3, Alpha-actinin skeletal muscle isoform 3, F-actin cross-linking protein) (PE)

Cat. Num. AAA6271353

• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ACTN3; Polyclonal Antibody; ID (ACTN3; Alpha-actinin-3; Alpha-actinin skeletal muscle isoform 3; F-actin cross-linking protein) (PE); F-actin cross-linking protein; anti-ACTN3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-ACTN3 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: ACTN3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 408-437 amino acids from the Central region of human ACTN3.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(ACTN3 Antibody (Center) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the ACTN3 antibody detected the ACTN3 protein (arrow).)

Immunohistochemistry (IHC)

(ACTN3 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ACTN3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-ACTN3 antibody

Alpha-actinin is an actin-binding protein with multiple roles in different cell types. This gene expression is limited to skeletal muscle. It is localized to the Z-disc and analogous dense bodies, where it helps to anchor the myofibrillar actin filaments

Product Categories/Family for anti-ACTN3 antibody

Antibodies; Actin Related Proteins

References

Rodriguez-Romo, G., et al. Eur. J. Appl. Physiol. 110(6):1099-1106(2010). Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Berman, Y., et al. Physiology (Bethesda) 25(4):250-259(2010). Ruiz, J.R., et al. Scand J Med Sci Sports (2010). In press: Buxens, A., et al. Scand J Med Sci Sports (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 4557241
• NCBI GeneID: 89
• NCBI Accession #: NP_001095.1
• NCBI GenBank Nucleotide #: NM_001104.2
• UniProt Accession #: Q08043; Q4KKV2; A6NP77
• Molecular Weight: 103,241 Da
• NCBI Official Full Name: alpha-actinin-3 isoform 1
• NCBI Official Synonym Full Names: actinin, alpha 3 (gene/pseudogene)
• NCBI Official Symbol: ACTN3
• NCBI Protein Information: alpha-actinin-3; F-actin cross-linking protein; alpha-actinin skeletal muscle
• UniProt Protein Name: Alpha-actinin-3
• Protein Family: Alpha-actinin
• UniProt Gene Name: ACTN3
• UniProt Entry Name: ACTN3_HUMAN

NCBI Description

This gene encodes a member of the alpha-actin binding protein gene family. The encoded protein is primarily expressed in skeletal muscle and functions as a structural component of sarcomeric Z line. This protein is involved in crosslinking actin containing thin filaments. An allelic polymorphism in this gene results in both coding and non-coding variants; the reference genome represents the coding allele. The non-functional allele of this gene is associated with elite athlete status. [provided by RefSeq, Feb 2014]

Uniprot Description

ACTN3: F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Belongs to the alpha-actinin family.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 11q13.1

Cellular Component: focal adhesion; actin filament; pseudopodium; cytosol

Molecular Function: integrin binding; protein binding; protein homodimerization activity; structural constituent of muscle; calcium ion binding; actin binding

Biological Process: regulation of apoptosis; focal adhesion formation; muscle filament sliding