Rabbit anti-Human ACTN1 Polyclonal Antibody | anti-ACTN1 antibody

ACTN1, NT (Alpha-actinin-1, Alpha-actinin Cytoskeletal Isoform, Non-muscle alpha-actinin-1, F-actin Cross-linking Protein) (FITC)

Cat. Num. AAA6461238

• Gene Names: ACTN1; BDPLT15
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot, Immunohistochemistry
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: ACTN1; Polyclonal Antibody; NT (Alpha-actinin-1; Alpha-actinin Cytoskeletal Isoform; Non-muscle alpha-actinin-1; F-actin Cross-linking Protein) (FITC); anti-ACTN1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ACTN1.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-ACTN1 antibody: FLISA, Flow Cytometry (FC/FACS), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1-30 in the N-terminal region of human ACTN1, UniProt Accession #NP_001093.1, P12814. Species sequence homology: bovine, mouse, monkey and rat
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in MDA-MB231 cell line lysates (35ug/lane) usingMBS629597. ACTN1 (arrow) was detected usingMBS629597.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human breast carcinoma usingMBS629597, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS629597 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of MDA-231 cells using MBS629597 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-ACTN1 antibody

ACTN1 is a F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.

Product Categories/Family for anti-ACTN1 antibody

Antibodies; Actin Related Proteins

References

1. Zhang, Z., et.al., J. Biol. Chem. 281(3): 1746-1754 (2006).

NCBI and Uniprot Product Information

• NCBI GI #: 4501891
• NCBI GeneID: 87
• NCBI Accession #: NP_001093.1
• NCBI GenBank Nucleotide #: NM_001102.3
• UniProt Accession #: P12814; Q1HE25; Q9BTN1; B3V8S3; B4DHH3
• Molecular Weight: 103,058 Da
• NCBI Official Full Name: alpha-actinin-1 isoform b
• NCBI Official Synonym Full Names: actinin, alpha 1
• NCBI Official Symbol: ACTN1
• NCBI Official Synonym Symbols: BDPLT15
• NCBI Protein Information: alpha-actinin-1; actinin 1 smooth muscle; non-muscle alpha-actinin-1; F-actin cross-linking protein; alpha-actinin cytoskeletal isoform
• UniProt Protein Name: Alpha-actinin-1
• Protein Family: Alpha-actinin
• UniProt Gene Name: ACTN1
• UniProt Entry Name: ACTN1_HUMAN

NCBI Description

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ACTN1: a cytoskeletal protein of the spectrin superfamily. An actin-binding protein with multiple roles in different cell types. A nonmuscle isoform structurally similar to the erythroid beta spectrin gene.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 14q24|14q22-q24

Cellular Component: extracellular space; cortical actin cytoskeleton; focal adhesion; dendritic spine; extracellular region; fascia adherens; pseudopodium; cytosol; Z disc; ruffle; cell projection; cytoplasm; plasma membrane; stress fiber; nucleus

Molecular Function: integrin binding; actin filament binding; protein domain specific binding; protein binding; ligand-dependent nuclear receptor transcription coactivator activity; double-stranded RNA binding; calcium ion binding; vinculin binding

Biological Process: actin crosslink formation; regulation of apoptosis; focal adhesion formation; actin filament bundle formation; platelet activation; extracellular matrix organization and biogenesis; platelet degranulation; negative regulation of cell motility; blood coagulation

Disease: Bleeding Disorder, Platelet-type, 15