Western Blot (WB)
(ACTL6B antibody (pAb) tested by Western blot. Whole cell extract of SH-SY5Y cells (12 ug) probed with ACTL6B antibody (pAb) at a dilution of 1:500.)
Rabbit anti-Human ACTL6B Polyclonal Antibody | anti-ACTL6B antibody
ACTL6B antibody (pAb)
Gene Names
ACTL6B; ACTL6; BAF53B
Reactivity
Human
Applications
Western Blot
Purity
Affinity Purified
Synonyms
ACTL6B; Polyclonal Antibody; ACTL6B antibody (pAb); ACTL6; BAF53B; anti-ACTL6B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-ACTL6B antibody
Western Blot (WB)
Application Notes
Applications Validated:
WB: 1:250-1:500 dilution
WB: 1:250-1:500 dilution
Immunogen
This antibody was raised against a peptide within the N-terminal region of human ACTL6B.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Western Blot (WB)
(ACTL6B antibody (pAb) tested by Western blot. Whole cell extract of SH-SY5Y cells (12 ug) probed with ACTL6B antibody (pAb) at a dilution of 1:500.)
Western Blot (WB)
(ACTL6B antibody (pAb) tested by Western blot. Whole cell extract of SH-SY5Y cells (12 ug) probed with ACTL6B antibody (pAb) at a dilution of 1:500.)
Related Product Information for anti-ACTL6B antibody
Short Description: Our ACTL6B antibody (pAb) was raised in a Rabbit host. It has been validated for use in Western blot; it has been shown to react with Human samples.
Background: ACTL6B (Actin-Like 6B) Belongs to the chromatin remodeling brain-specific BAF (bBAF) complex, as such plays a role in remodeling mononucleosomes in an ATP-dependent fashion. Belongs to the neuron-specific chromatin remodeling complex (nBAF complex) and is required for postmitotic neural development and dendritic outgrowth. During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. ACTL6B/BAF53B is not essential for assembly of the nBAF complex but is required for targeting the complex and CREST to the promoter of genes essential for dendritic growth (By similarity).
Background: ACTL6B (Actin-Like 6B) Belongs to the chromatin remodeling brain-specific BAF (bBAF) complex, as such plays a role in remodeling mononucleosomes in an ATP-dependent fashion. Belongs to the neuron-specific chromatin remodeling complex (nBAF complex) and is required for postmitotic neural development and dendritic outgrowth. During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. ACTL6B/BAF53B is not essential for assembly of the nBAF complex but is required for targeting the complex and CREST to the promoter of genes essential for dendritic growth (By similarity).
Product Categories/Family for anti-ACTL6B antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
46,877 Da
NCBI Official Full Name
actin-like protein 6B
NCBI Official Synonym Full Names
actin-like 6B
NCBI Official Symbol
ACTL6B
NCBI Official Synonym Symbols
ACTL6; BAF53B
NCBI Protein Information
actin-like protein 6B; 53 kDa BRG1-associated factor B; BRG1-associated factor 53B; actin-like 6; actin-related protein Baf53b; arpNalpha; hArpN alpha
UniProt Protein Name
Actin-like protein 6B
Protein Family
UniProt Gene Name
ACTL6B
UniProt Synonym Gene Names
ACTL6; BAF53B; BAF53B
UniProt Entry Name
ACL6B_HUMAN
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Product Notes
The ACTL6B actl6b (Catalog #AAA389012) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ACTL6B antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ACTL6B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications Validated: WB: 1:250-1:500 dilution. Researchers should empirically determine the suitability of the ACTL6B actl6b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ACTL6B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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