Rabbit anti-Human Actin-like protein 6B Polyclonal Antibody | anti-ACTL6B antibody

Rabbit anti-human Actin-like protein 6B polyclonal Antibody(ACTL6B)

Cat. Num. AAA1498141

• Gene Names: ACTL6B; ACTL6; BAF53B; arpNalpha
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Antigen Affinity Purified
• Synonyms: Actin-like protein 6B; Polyclonal Antibody; Rabbit anti-human Actin-like protein 6B polyclonal Antibody(ACTL6B); 53 kDa BRG1-associated factor B; Actin-related protein Baf53b; ArpNalpha; BRG1-associated factor 53B; BAF53B; ACTL6B; ACTL6; anti-ACTL6B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purified
• Sequence Length: 426

• Applicable Applications for anti-ACTL6B antibody: ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: Recommended dilution: IHC:1:20-1:200
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Conjugate: Non-conjugated
• Immunogen: Recombinant human Actin-like protein 6B protein(1-280aa)
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-323503
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human skin using MBS1498141 at dilution 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human brain using MBS1498141 at dilution 1:100)

Related Product Information for anti-ACTL6B antibody

Belongs to the chromatin remodeling brain-specific BAF (bBAF) complex, as such plays a role in remodeling mononucleosomes in an ATP-dependent fashion. Belongs to the neuron-specific chromatin remodeling complex (nBAF complex) and is required for postmitotic neural development and dendritic outgrowth. During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. ACTL6B/BAF53B is not essential for assembly of the nBAF complex but is required for targeting the complex and CREST to the promoter of genes essential for dendritic growth.

NCBI and Uniprot Product Information

• NCBI GI #: 7705294
• NCBI GeneID: 51412
• NCBI Accession #: NP_057272.1
• NCBI GenBank Nucleotide #: NM_016188.4
• UniProt Accession #: O94805; O75421; A4D2D0
• Molecular Weight: 46,877 Da
• NCBI Official Full Name: actin-like protein 6B
• NCBI Official Synonym Full Names: actin like 6B
• NCBI Official Symbol: ACTL6B
• NCBI Official Synonym Symbols: ACTL6; BAF53B; arpNalpha
• NCBI Protein Information: actin-like protein 6B
• UniProt Protein Name: Actin-like protein 6B
• Protein Family: Actin-like protein
• UniProt Gene Name: ACTL6B
• UniProt Synonym Gene Names: ACTL6; BAF53B; BAF53B
• UniProt Entry Name: ACL6B_HUMAN

NCBI Description

The protein encoded by this gene is a member of a family of actin-related proteins (ARPs) which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a subunit of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. This subunit may be involved in the regulation of genes by structural modulation of their chromatin, specifically in the brain. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]

Uniprot Description

BAF53B: a protein of the actin family involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Required for maximal ATPase activity of the helicase SMARCA4 and for association of the SMARCA4 containing remodelling complex BAF with chromatin/nuclear matrix.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 7q22

Cellular Component: nucleolus; nucleus; SWI/SNF complex

Molecular Function: structural constituent of cytoskeleton; transcription coactivator activity

Biological Process: chromatin remodeling; establishment and/or maintenance of chromatin architecture; regulation of transcription from RNA polymerase II promoter; spinal cord development; transcription, DNA-dependent