Rabbit ACSS2 Polyclonal Antibody | anti-ACSS2 antibody

ACSS2 antibody

Cat. Num. AAA9405873

• Gene Names: ACSS2; ACS; ACSA; ACAS2; ACECS; dJ1161H23.1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: ACSS2; Polyclonal Antibody; ACSS2 antibody; ACS; ACSA; ACAS2; ACECS; dJ1161H23.1; anti-ACSS2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total ACSS2 antibody.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 714

• Applicable Applications for anti-ACSS2 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunofluorescence: 1:50 - 1:200
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human ACSS2.
• Target Name: ACSS2
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using ACSS2 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cell using ACSS2 antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-ACSS2 antibody

This gene encodes a cytosolic enzyme that catalyzes the activation of acetate for use in lipid synthesis and energy generation. The protein acts as a monomer and produces acetyl-CoA from acetate in a reaction that requires ATP. Expression of this gene is regulated by sterol regulatory element-binding proteins, transcription factors that activate genes required for the synthesis of cholesterol and unsaturated fatty acids. Alternative splicing results in multiple transcript variants.

Product Categories/Family for anti-ACSS2 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 253970489
• NCBI GeneID: 55902
• NCBI Accession #: NP_001070020.2
• NCBI GenBank Nucleotide #: NM_001076552.2
• UniProt Accession #: Q9NR19; Q5QPH2; Q5QPH3; Q8N238; Q96EL0; Q9NQP7; Q9UJ15; A6NE90
• Molecular Weight: 80,087 Da
• NCBI Official Full Name: acetyl-coenzyme A synthetase, cytoplasmic isoform 2
• NCBI Official Synonym Full Names: acyl-CoA synthetase short-chain family member 2
• NCBI Official Symbol: ACSS2
• NCBI Official Synonym Symbols: ACS; ACSA; ACAS2; ACECS; dJ1161H23.1
• NCBI Protein Information: acetyl-coenzyme A synthetase, cytoplasmic
• UniProt Protein Name: Acetyl-coenzyme A synthetase, cytoplasmic
• Protein Family: Acetyl-coenzyme A synthetase
• UniProt Gene Name: ACSS2
• UniProt Synonym Gene Names: ACAS2; ACS; AceCS
• UniProt Entry Name: ACSA_HUMAN

NCBI Description

This gene encodes a cytosolic enzyme that catalyzes the activation of acetate for use in lipid synthesis and energy generation. The protein acts as a monomer and produces acetyl-CoA from acetate in a reaction that requires ATP. Expression of this gene is regulated by sterol regulatory element-binding proteins, transcription factors that activate genes required for the synthesis of cholesterol and unsaturated fatty acids. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2009]

Uniprot Description

ACSS2: Activates acetate so that it can be used for lipid synthesis or for energy generation. Monomer. Belongs to the ATP-dependent AMP-binding enzyme family.

Protein type: Carbohydrate Metabolism - pyruvate; EC 6.2.1.1; Carbohydrate Metabolism - propanoate; Ligase; Carbohydrate Metabolism - glycolysis and gluconeogenesis

Chromosomal Location of Human Ortholog: 20q11.22

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; cytoplasm; cytosol

Molecular Function: acetate-CoA ligase activity; ATP binding; AMP binding

Biological Process: acetyl-CoA biosynthetic process from acetate; xenobiotic metabolic process; lipid biosynthetic process; propionate biosynthetic process; ethanol oxidation; acetate biosynthetic process