Rabbit anti-Human ACSL1 Polyclonal Antibody | anti-ACSL1 antibody

Anti-ACSL1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8102990

• Gene Names: ACSL1; ACS1; LACS; FACL1; FACL2; LACS1; LACS2
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: ACSL1; Polyclonal Antibody; Anti-ACSL1 Antibody; Rabbit Polyclonal; ACSL1 Antibody; Rabbit PAb; Antigen Affinity Purified; acyl-CoA synthetase long-chain family member 1; Anti-ACS1 Antibody; Anti-FACL1 Antibody; Anti-FACL2 Antibody; Anti-LACS Antibody; Anti-LACS1 Antibody; Anti-LACS2 Antibody; anti-ACSL1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human ACSL1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-ACSL1 antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:200-1:1000
• Immunogen: A synthetic peptide corresponding to the center region of the Human ACSL1
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human ACSL1, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human ACSL1 in human kidney with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human ACSL1 in human hepatoma with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)

Related Product Information for anti-ACSL1 antibody

Long-chain acyl-CoA synthetases (ACSLs), which are responsible for activation of the most abundant long-chain fatty acids, are commonly deregulated in cancer. This deregulation is also associated with poor survival in patients with cancer. Fatty acids physiologically regulate ACSL expression, but cancer cells could hijack certain involved regulatory mechanisms to deregulate ACSLs. Among the five family isoforms, ACSL1 and ACSL4 are able to promote ungoverned cell growth, facilitate tumor invasion and evade programmed cell death. Acyl-CoA synthetase long-chain family member 1 (ACSL1), which are involved in altered lipid metabolism, lipolysis, and de novo FA synthesis in highly aggressive prostate and breast cancer cells.Global cardiac transcriptional analysis revealed differential expression of genes involved in cardiac metabolism, fibrosis, and hypertrophy development in Acsl1H-/-hearts 2 weeks after Acsl1 ablation.Short-term cardiac-specific ACSL1 inactivation resulted in metabolic and transcriptional derangements distinct from those observed upon complete ACSL1 knockout, suggesting heart-specific mTOR (mechanistic target of rapamycin) signaling that occurs during the early stages of substrate switching. The hypertrophy observed with partial Acsl1 ablation occurs in the context of normal cardiac function and is reminiscent of a physiological process, making this a useful model to study the transition from physiological to pathological hypertrophy.

NCBI and Uniprot Product Information

• NCBI GI #: 417241
• NCBI GeneID: 2180
• UniProt Accession #: P33121; P41215; Q8N8V7; Q8TA99; B7Z452; D3DP57
• Molecular Weight: 698
• NCBI Official Full Name: Long-chain-fatty-acid--CoA ligase 1
• NCBI Official Synonym Full Names: acyl-CoA synthetase long-chain family member 1
• NCBI Official Symbol: ACSL1
• NCBI Official Synonym Symbols: ACS1; LACS; FACL1; FACL2; LACS1; LACS2
• NCBI Protein Information: long-chain-fatty-acid--CoA ligase 1; LACS 1; LACS 2; acyl-CoA synthetase 1; palmitoyl-CoA ligase 1; palmitoyl-CoA ligase 2; paltimoyl-CoA ligase 1; lignoceroyl-CoA synthase; long-chain acyl-CoA synthetase 1; long-chain acyl-CoA synthetase 2; long-chain fa
• UniProt Protein Name: Long-chain-fatty-acid--CoA ligase 1
• Protein Family: Long-chain-fatty-acid--CoA ligase
• UniProt Gene Name: ACSL1
• UniProt Synonym Gene Names: FACL1; FACL2; LACS; LACS1; LACS2; ACS1; LACS 1; LACS 2
• UniProt Entry Name: ACSL1_HUMAN

NCBI Description

The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]

Uniprot Description

ACSL1: Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses palmitoleate, oleate and linoleate. Highly expressed in liver, heart, skeletal muscle, kidney and erythroid cells, and to a lesser extent in brain, lung, placenta and pancreas. Belongs to the ATP-dependent AMP-binding enzyme family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - fatty acid; Ligase; Membrane protein, integral; EC 6.2.1.3

Chromosomal Location of Human Ortholog: 4q35.1

Cellular Component: peroxisomal membrane; mitochondrial outer membrane; endoplasmic reticulum membrane; mitochondrion; membrane; plasma membrane; integral to membrane

Molecular Function: ATP binding; long-chain-fatty-acid-CoA ligase activity

Biological Process: response to drug; response to oleate; unsaturated fatty acid metabolic process; lipid biosynthetic process; linoleic acid metabolic process; adiponectin-mediated signaling pathway; triacylglycerol biosynthetic process; cellular lipid metabolic process; long-chain fatty acid metabolic process; response to organic cyclic substance; response to nutrient; xenobiotic catabolic process