Rabbit anti-Human ACR Polyclonal Antibody | anti-ACR antibody

ACR, CT (ACR, ACRS, Acrosin, Acrosin light chain, Acrosin heavy chain) (AP)

Cat. Num. AAA6271046

• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: ACR; Polyclonal Antibody; CT (ACR; ACRS; Acrosin; Acrosin light chain; Acrosin heavy chain) (AP); Acrosin heavy chain; anti-ACR antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ACR
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.

• Applicable Applications for anti-ACR antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: ACR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 392~421 amino acids from the C-terminal region of human ACR.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Related Product Information for anti-ACR antibody

ACR is the major proteinase present in the acrosome of mature spermatozoa. It is a typical serine proteinase with trypsin-like specificity. It is stored in the acrosome in its precursor form, proacrosin. The active enzyme functions in the lysis of the zona pellucida, thus facilitating penetration of the sperm through the innermost glycoprotein layers of the ovum. The mRNA for proacrosin is synthesized only in the postmeiotic stages of spermatogenesis. In humans proacrosin first appears in the haploid spermatids.

Product Categories/Family for anti-ACR antibody

Antibodies; Enzymes; Protease (Proteinase; Peptidase)

References

Dube, C., et.al., J. Androl. 26 (4), 519-528 (2005). Furlong, L.I., et.al., Fertil. Steril. 83 (6), 1791-1796 (2005).

NCBI and Uniprot Product Information

• NCBI GI #: 148613878
• NCBI GeneID: 49
• NCBI Accession #: NP_001088.2
• NCBI GenBank Nucleotide #: NM_001097.2
• UniProt Accession #: P10323; Q6ICK2
• Molecular Weight: 45,847 Da
• NCBI Official Full Name: acrosin
• NCBI Official Synonym Full Names: acrosin
• NCBI Official Symbol: ACR
• NCBI Protein Information: acrosin; proacrosin; preproacrosin; acrosin light and heavy chain prepropeptide
• UniProt Protein Name: Acrosin
• Protein Family: Acrosin
• UniProt Gene Name: ACR
• UniProt Synonym Gene Names: ACRS
• UniProt Entry Name: ACRO_HUMAN

NCBI Description

Acrosin is the major proteinase present in the acrosome of mature spermatozoa. It is a typical serine proteinase with trypsin-like specificity. It is stored in the acrosome in its precursor form, proacrosin. The active enzyme functions in the lysis of the zona pellucida, thus facilitating penetration of the sperm through the innermost glycoprotein layers of the ovum. The mRNA for proacrosin is synthesized only in the postmeiotic stages of spermatogenesis. In humans proacrosin first appears in the haploid spermatids. [provided by RefSeq, Jul 2008]

Uniprot Description

ACR: Acrosin is the major protease of mammalian spermatozoa. It is a serine protease of trypsin-like cleavage specificity, it is synthesized in a zymogen form, proacrosin and stored in the acrosome. Belongs to the peptidase S1 family.

Protein type: Protease; EC 3.4.21.10

Chromosomal Location of Human Ortholog: 22q13.33

Cellular Component: protein complex; extracellular region; Golgi-associated vesicle; nucleus; acrosomal matrix

Molecular Function: mannose binding; protein binding; copper ion binding; amidase activity; DNA binding; zinc ion binding; serine-type endopeptidase activity; drug binding; fucose binding

Biological Process: response to steroid hormone stimulus; binding of sperm to zona pellucida; single fertilization; acrosome reaction; adenylate cyclase activation; penetration of zona pellucida; acrosome matrix dispersal; multicellular organism reproduction