Rabbit ACPP Polyclonal Antibody | anti-ACPP antibody

ACPP Antibody

Cat. Num. AAA9607361

• Gene Names: ACPP; ACP3; 5'-NT; ACP-3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: ACPP; Polyclonal Antibody; ACPP Antibody; 5'-NT; 5'-nucleotidase; Acid phosphatase prostate; ACP 3; ACP3; acpP; Ecto-5'-nucleotidase; PAP; PPAP_HUMAN; Prostatic acid phosphatase; Prostatic acid phosphotase; Thiamine monophosphatase; TMPase; anti-ACPP antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ACPP antibody detects endogenous levels of total ACPP
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 386

• Applicable Applications for anti-ACPP antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: A synthesized peptide derived from human ACPP
• Subcellular Location: Secreted and Lysosome Membrane. Predominantly localized in the plasma membrane but also detected in intracellular vesicles.
• Tissue Specificity: Highly expressed in the prostate, restricted to glandular and ductal epithelial cells. Also expressed in bladder, kidney, pancreas, lung, cervix, testis and ovary. Weak expression in a subset of pancreatic islet cells, squamous epithelia, the pilosebaceous unit, colonic neuroendocrine cells and skin adnexal structures. Isoform 2 also expressed in the sarcolemma of skeletal muscle. Levels of this cellular isoform decreased in prostate cancer.
• Predicted Cross Reactivity: Pig, Bovine, Horse, Sheep, Dog
• Similarity: Pig (86%), Bovine (86%), Horse (86%), Sheep (86%), Dog (86%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts of MCF-7, using ACPP antibody. The lane on the left is treated with the antigen-specific peptide.)

Immunohistochemistry (IHC)

(MBS9607361 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Related Product Information for anti-ACPP antibody

Description: ACPP is also named as PAP(prostatic acid phosphatase), 5'-nucleotidase, thiamine monophosphatas and Belongs to the histidine acid phosphatase family.This enzyme is a homodimeric glycoprotein with a subunit molecular mass of 50 kDa and is an androgen-stimulated secretory product of the human prostate gland(PMID:8132635).It is also a non-specific phosphatase and has been implicated in the progression of prostate cancer, which inactivates LPA in human seminal plasma(PMID:15280042). This protein has three glycosylation sites(PMID:10639192; 12525165). This antibody is specific to ACPP.
Function: A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma.
Subunit Structure: Homodimer; dimer formation is required for phosphatase activity.
Post-translational Modifications: N-glycosylated. High mannose content, partially sialylated and fucosylated biantennary complex. Also fucosylated with partially sialylated triantennary complex oligosaccharides. Proteolytically cleaved in seminal fluid to produce several peptides. Peptide PAPf39, the most prominent, forms amyloid beta-sheet fibrils, SEVI (semen-derived enhancer of viral infection) which entrap HIV virions, attach them to target cells and enhance infection. SEVI amyloid fibrils are degraded by polyphenol epigallocatechin-3-gallate (EGCG), a constituent of green tea. Target cell attachment and enhancement of HIV infection is inhibited by surfen. Also similarly boosts XMRV (xenotropic murine leukemia virus-related virus) infection.
Similarity: Belongs to the histidine acid phosphatase family.

NCBI and Uniprot Product Information

• NCBI GI #: 6382064
• NCBI GeneID: 55
• NCBI Accession #: NP_001090.2
• NCBI GenBank Nucleotide #: NM_001099.4
• UniProt Accession #: P15309; Q5FBY0; Q96KY0; Q96QK9; Q96QM0; D3DNC6
• Molecular Weight: Observed: 48 kDa; Predicted: 45 kDa
• NCBI Official Full Name: prostatic acid phosphatase isoform PAP
• NCBI Official Synonym Full Names: acid phosphatase, prostate
• NCBI Official Symbol: ACPP
• NCBI Official Synonym Symbols: ACP3; 5'-NT; ACP-3
• NCBI Protein Information: prostatic acid phosphatase
• UniProt Protein Name: Prostatic acid phosphatase
• Protein Family: Acyl carrier protein
• UniProt Gene Name: ACPP
• UniProt Synonym Gene Names: PAP; 5'-NT; TMPase

NCBI Description

This gene encodes an enzyme that catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is synthesized under androgen regulation and is secreted by the epithelial cells of the prostate gland. An alternatively spliced transcript variant encoding a longer isoform has been found for this gene. This isoform contains a transmembrane domain and is localized in the plasma membrane-endosomal-lysosomal pathway. [provided by RefSeq, Sep 2008]

Uniprot Description

A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma.