Rabbit anti-Human ACP5 Polyclonal Antibody | anti-ACP5 antibody

ACP5, NT (ACP5, Tartrate-resistant acid phosphatase type 5, Tartrate-resistant acid ATPase, Type 5 acid phosphatase) (FITC)

Cat. Num. AAA6271027

• Gene Names: ACP5; TRAP; SPENCDI
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ACP5; Polyclonal Antibody; NT (ACP5; Tartrate-resistant acid phosphatase type 5; Tartrate-resistant acid ATPase; Type 5 acid phosphatase) (FITC); Type 5 acid phosphatase; anti-ACP5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-ACP5 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ACP5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 75-101 amino acids from the N-terminal region of human ACP5.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ACP5 Antibody (N-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the ACP5 antibody detected the ACP5 protein (arrow).)

Related Product Information for anti-ACP5 antibody

This gene encodes an iron containing glycoprotein which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate. [provided by RefSeq].

Product Categories/Family for anti-ACP5 antibody

Antibodies; Enzymes; Phosphatase

References

Shih, K.C., et al. Metab. Clin. Exp. 59(1):144-151(2010). Wu, Y.Y., et al. BMC Cancer 10, 158 (2010): Baranzini, S.E., et al. Hum. Mol. Genet. 18(4):767-778(2009). Qin, Y.J., et al. Acta Pharmacol. Sin. 29(12):1493-1498(2008). de Souza Malaspina, T.S., et al. J. Mol. Histol. 39(6):627-634(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 4501873
• NCBI GeneID: 54
• NCBI Accession #: NP_001602.1
• NCBI GenBank Nucleotide #: NM_001611.3
• UniProt Accession #: P13686; Q2TAB1; Q6IAS6; Q9UCJ5; Q9UCJ6; Q9UCJ7; A8K3V2
• NCBI Official Full Name: tartrate-resistant acid phosphatase type 5
• NCBI Official Synonym Full Names: acid phosphatase 5, tartrate resistant
• NCBI Official Symbol: ACP5
• NCBI Official Synonym Symbols: TRAP; SPENCDI
• NCBI Protein Information: tartrate-resistant acid phosphatase type 5; TrATPase; tartrate-resistant acid ATPase
• UniProt Protein Name: Tartrate-resistant acid phosphatase type 5
• Protein Family: Acyl carrier protein
• UniProt Gene Name: ACP5
• UniProt Synonym Gene Names: TR-AP; TrATPase
• UniProt Entry Name: PPA5_HUMAN

NCBI Description

This gene encodes an iron containing glycoprotein which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate. [provided by RefSeq, Aug 2008]

Uniprot Description

ACP5: Involved in osteopontin/bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias. Defects in ACP5 are the cause of spondyloenchondrodysplasia with immune dysregulation (SPENCDI). A disease characterized by vertebral and metaphyseal dysplasia, spasticity with cerebral calcifications, and strong predisposition to autoimmune diseases. The skeletal dysplasia is characterized by radiolucent and irregular spondylar and metaphyseal lesions that represent islands of chondroid tissue within bone. ACP5 inactivating mutations result in a functional excess of phosphorylated osteopontin causing deregulation of osteopontin signaling and consequential autoimmune disease. Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.

Protein type: Motility/polarity/chemotaxis; Phosphatase; EC 3.1.3.2; Cofactor and Vitamin Metabolism - riboflavin

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: lysosome; integral to membrane; cytosol

Molecular Function: acid phosphatase activity; ferric iron binding; ferrous iron binding

Biological Process: negative regulation of interleukin-12 production; vitamin metabolic process; negative regulation of nitric oxide biosynthetic process; response to lipopolysaccharide; negative regulation of interleukin-1 beta production; negative regulation of superoxide release; negative regulation of tumor necrosis factor production; riboflavin metabolic process; defense response to Gram-positive bacterium; dephosphorylation; response to cytokine stimulus; negative regulation of inflammatory response; water-soluble vitamin metabolic process; bone resorption

Disease: Spondyloenchondrodysplasia With Immune Dysregulation