Rabbit ACKR3 / CXCR7 / RDC1 Polyclonal Antibody | anti-ACKR3 antibody

Rabbit Polyclonal (IgG) to Human ACKR3 / CXCR7

Cat. Num. AAA241706

• Gene Names: ACKR3; RDC1; CXCR7; RDC-1; CMKOR1; CXC-R7; CXCR-7; GPR159
• Reactivity: Bat, Gibbon, Bovine, Dog, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat; Predicted Reactivity: Xenopus (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Protein G Purified
• Synonyms: ACKR3 / CXCR7 / RDC1; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human ACKR3 / CXCR7; Anti-ACKR3 / CXCR7 / RDC1 Antibody (aa100-150) IHC-plus; ACKR3; Chemokine orphan receptor 1; CMKOR1; CXCR7; CXC-R7; GPRN1; RDC1; RDC-1; CXCR-7; G-protein coupled receptor 159; GPR159; Human ACKR3; anti-ACKR3 antibody

• Host: Rabbit
• Reactivity: Bat, Gibbon, Bovine, Dog, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat; Predicted Reactivity: Xenopus (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: A portion of amino acids 100-150 of human RDC1
• Purity/Purification: Protein G Purified
• Form/Format: PBS, 0.05% BSA, 0.05% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 362

• Applicable Applications for anti-ACKR3 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Flo (1 - 10 ug/ml), IHC-P (10 ug/ml), WB (4 - 6 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: A synthetic peptide (QHNQWPMGELTC) corresponding amino acids 106-117 of human CXCR7/RDC1 was used as the immunogen, GenBank NP_064707.1.
• Immunogen Type: Synthetic peptide
• Immunogen: ACKR3 / CXCR7 antibody was raised against a synthetic peptide (QHNQWPMGELTC) corresponding amino acids 106-117 of human CXCR7/RDC1 was used as the immunogen, GenBank NP_064707.1. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Bat, Dog, Horse, Rabbit, Pig, Opossum (100%); Platypus, Xenopus (92%); Turkey (83%).
• Antigen Modification: aa100-150
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-CXCR7 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Western Blot (WB)

(Western blot of human CXCR7/RDC1 in Jurkat cell lysate in the 1) absence and 2) presence of immunizing peptide using at 5 ug/ml.)

Related Product Information for anti-ACKR3 antibody

RDC1 was originally considered to be an Adrenomedullin receptor that binds calcitonin gene-related peptide (CGRP) and adrenomedullin. RDC1 and GPRN1 represent two tissue-specific subtypes of the same 60-kD receptor. RDC1 has the structural characteristics of a chemokine receptor. Recently it has been shown that RDC1 binds to the chemokine CXCL12 and should be considered to be a chemokine receptor.

Product Categories/Family for anti-ACKR3 antibody

Family: GPCR; Subfamily: Chemokine

NCBI and Uniprot Product Information

• NCBI GI #: 31083344
• NCBI GeneID: 57007
• NCBI Accession #: NP_064707.1
• NCBI GenBank Nucleotide #: NM_020311.2
• UniProt Accession #: P25106; Q53RV4; Q8NE10; Q92938; Q92986; A8K6J4
• Molecular Weight: 41,493 Da
• NCBI Official Full Name: atypical chemokine receptor 3
• NCBI Official Synonym Full Names: atypical chemokine receptor 3
• NCBI Official Symbol: ACKR3
• NCBI Official Synonym Symbols: RDC1; CXCR7; RDC-1; CMKOR1; CXC-R7; CXCR-7; GPR159
• NCBI Protein Information: atypical chemokine receptor 3; G protein-coupled receptor; chemokine orphan receptor 1; G-protein coupled receptor 159; C-X-C chemokine receptor type 7; chemokine (C-X-C motif) receptor 7; G-protein coupled receptor RDC1 homolog
• UniProt Protein Name: Atypical chemokine receptor 3
• Protein Family: Atypical chemokine receptor
• UniProt Gene Name: ACKR3
• UniProt Synonym Gene Names: CMKOR1; CXCR7; GPR159; RDC1; CXC-R7; CXCR-7; RDC-1
• UniProt Entry Name: ACKR3_HUMAN

NCBI Description

This gene encodes a member of the G-protein coupled receptor family. Although this protein was earlier thought to be a receptor for vasoactive intestinal peptide (VIP), it is now considered to be an orphan receptor, in that its endogenous ligand has not been identified. The protein is also a coreceptor for human immunodeficiency viruses (HIV). Translocations involving this gene and HMGA2 on chromosome 12 have been observed in lipomas. [provided by RefSeq, Jul 2008]

Uniprot Description

CMKOR1: Receptor for chemokines CXCL12/SDF1 and CXCL11. Does not elicit classical chemokine receptor signaling; chemokine binding does not activate G-protein-mediated signal transduction but instead induces beta-arrestin recruitment, leading to ligand internalization and activation of MAPK signaling pathway. Acts as a scavenger for CXCL12/SDF1 and, to a lesser extent, for CXCL11. Required for regulation of CXCR4 protein levels in migrating interneurons, thereby adapting their chemokine responsiveness. In glioma cells, transduces signals via MEK/ERK pathway, mediating resistance to apoptosis. Promotes cell growth and survival. Not involved in cell migration, adhesion or proliferation of normal hematopoietic progenitors but activated by CXCL11 in malignant hemapoietic cells, leading to phosphorylation of ERK1/2 (MAPK3/MAPK1) and enhanced cell adhesion and migration. Plays a regulatory role in CXCR4-mediated activation of cell surface integrins by CXCL12. Required for heart valve development. Acts as coreceptor with CXCR4 for a restricted number of HIV isolates. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, integral; GPCR, family 1; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: recycling endosome; cell surface; perinuclear region of cytoplasm; early endosome; integral to membrane; plasma membrane; coated pit; endosome

Molecular Function: C-X-C chemokine binding; protein binding; coreceptor activity; scavenger receptor activity; C-X-C chemokine receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; viral reproduction; receptor internalization; angiogenesis; cell adhesion; vasculogenesis; chemotaxis