Rabbit anti-Human ACK1 Polyclonal Antibody | anti-ACK1 antibody

ACK1, CT (Tyrosine Kinase Non-receptor Protein 2, Activated CDC42 Kinase 1, ACK-1, TNK2) (Azide free) (HRP)

Cat. Num. AAA6461066

• Gene Names: TNK2; ACK; ACK1; ACK-1; p21cdc42Hs
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: ACK1; Polyclonal Antibody; CT (Tyrosine Kinase Non-receptor Protein 2; Activated CDC42 Kinase 1; ACK-1; TNK2) (Azide free) (HRP); anti-ACK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ACK1.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-ACK1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1000; WB: 1:10-1:50; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide corresponding to a portion of amino acids within residues 939-969 in the C-terminal region of human ACK1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-ACK1 Antibody (C-term) in CEM cell line lysates (35ug/lane). ACK1(arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of ACK1 (arrow) using rabbit polyclonal ACK1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ACK1 gene (Lane 2) (60 KD recombinant protein).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(ACK1 Antibody (C-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the ACK1 antibody detected the ACK1 protein (arrow).)

Product Categories/Family for anti-ACK1 antibody

Antibodies; Protein Kinases

References

1. Yokoyama, N., et al., J. Biol. Chem. 278(48):47713-47723 (2003). 2. Manser, E., et al., Nature 363(6427):364-367 (1993).

NCBI and Uniprot Product Information

• NCBI GI #: 58331191
• NCBI GeneID: 10188
• NCBI Accession #: NP_001010938.1
• NCBI GenBank Nucleotide #: NM_001010938.1
• UniProt Accession #: Q07912; Q6ZMQ0; Q8N6U7; Q96H59
• Molecular Weight: 114,569 Da
• NCBI Official Full Name: activated CDC42 kinase 1 isoform 2
• NCBI Official Synonym Full Names: tyrosine kinase, non-receptor, 2
• NCBI Official Symbol: TNK2
• NCBI Official Synonym Symbols: ACK; ACK1; ACK-1; p21cdc42Hs
• NCBI Protein Information: activated CDC42 kinase 1; activated p21cdc42Hs kinase; activated Cdc42-associated kinase 1; tyrosine kinase non-receptor protein 2
• UniProt Protein Name: Activated CDC42 kinase 1
• Protein Family: Activator of C kinase protein
• UniProt Gene Name: TNK2
• UniProt Synonym Gene Names: ACK1; ACK-1
• UniProt Entry Name: ACK1_HUMAN

NCBI Description

This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

Ack: a tyrosine kinase that binds Cdc42 in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. May be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42 and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Amplification in primary tumors correlates with metastatic potential . Two differentially spliced isoforms have been described.

Protein type: Protein kinase, TK; EC 2.7.11.1; Kinase, protein; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); TK group; Ack family

Chromosomal Location of Human Ortholog: 3q29

Cellular Component: extrinsic to internal side of plasma membrane; adherens junction; growth cone; cytoplasmic vesicle membrane; cell soma; membrane; clathrin-coated vesicle; axon; cytoplasm; dendrite; plasma membrane; coated pit; nucleus; endosome

Molecular Function: protein serine/threonine kinase activity; protein binding; metal ion binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; protein serine/threonine/tyrosine kinase activity; WW domain binding; ATP binding; GTPase inhibitor activity; hormone receptor binding; epidermal growth factor receptor binding

Biological Process: positive regulation of peptidyl-tyrosine phosphorylation; cell migration; cell surface receptor linked signal transduction; small GTPase mediated signal transduction; negative regulation of catalytic activity; innate immune response; endocytosis; cell differentiation; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of cell proliferation