Human Arachidonate Lipoxygenase 3 (ALOXE3) ELISA Kit | ALOXE3 elisa kit

Human Arachidonate Lipoxygenase 3 (ALOXE3) ELISA Kit

Cat. Num. AAA451461

• Gene Names: ALOXE3; ARCI3; E-LOX; eLOX3; eLOX-3
• Reactivity: Human
• Synonyms: Arachidonate Lipoxygenase 3 (ALOXE3); Human Arachidonate Lipoxygenase 3 (ALOXE3) ELISA Kit; ALOX-E3; LOX3; eLOX3; E-LOX; Epidermis-Type Lipoxygenase 3; Hydroperoxy icosatetraenoate dehydratase Hydroperoxide isomerase ALOXE3; ALOXE3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ALOXE3. No significant cross-reactivity or interference between ALOXE3 and analogues was observed.
• Sequence Length: 843

• Samples: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.114ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ALOXE3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ALOXE3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ALOXE3 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ALOXE3 in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ALOXE3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ALOXE3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ALOXE3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ALOXE3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 260166612
• NCBI GeneID: 59344
• NCBI Accession #: NP_001159432.1
• NCBI GenBank Nucleotide #: NM_001165960.1
• UniProt Accession #: Q9BYJ1; Q3ZB74; Q9H4F2; Q9HC22; B2R981; B7Z3W0
• Molecular Weight: 95,186 Da
• NCBI Official Full Name: hydroperoxide isomerase ALOXE3 isoform 1
• NCBI Official Synonym Full Names: arachidonate lipoxygenase 3
• NCBI Official Symbol: ALOXE3
• NCBI Official Synonym Symbols: ARCI3; E-LOX; eLOX3; eLOX-3
• NCBI Protein Information: hydroperoxide isomerase ALOXE3
• UniProt Protein Name: Hydroperoxide isomerase ALOXE3
• Protein Family: Hydroperoxide isomerase
• UniProt Gene Name: ALOXE3
• UniProt Synonym Gene Names: Epidermal LOX-3; e-LOX-3; eLOX-3

NCBI Description

This gene is a member of the lipoxygenase family, which are catabolized by arachidonic acid-derived compounds. The encoded enzyme is a hydroperoxide isomerase that synthesizes a unique type of epoxy alcohol (8R-hydroxy-11R,12R-epoxyeicosa-5Z,9E,14Z-trienoic acid) from 12R-hydroperoxyeicosatetraenoic acid (12R-HPETE). This epoxy alcohol can activate the the nuclear receptor peroxisome proliferator-activated receptor alpha (PPARalpha), which is implicated in epidermal differentiation. Loss of function of the enzyme encoded by this gene results in ichthyosis, implicating the function of this gene in the differentiation of human skin. This gene is part of a cluster of lipoxygenase genes on 17p13.1. Mutations in this gene result in nonbullous congenital ichthyosiform erythroderma (NCIE). Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]

Uniprot Description

Non-heme iron-containing lipoxygenase which is atypical in that it displays a prominent hydroperoxide isomerase activity and a reduced dioxygenase activity compared to other lipoxygenases. The hydroperoxide isomerase activity catalyzes the isomerization of hydroperoxides, derived from arachidonic and linoleic acid by ALOX12B, into hepoxilin-type epoxyalcohols. The dioxygenase activity requires a step of activation of the enzyme by molecular oxygen. In presence of oxygen, oxygenates polyunsaturated fatty acids, including arachidonic acid, to produce fatty acid hydroperoxides. In the skin, acts downstream of ALOX12B on the linoleate moiety of esterified omega-hydroxyacyl-sphingosine (EOS) ceramides to produce an epoxy-ketone derivative, a crucial step in the conjugation of omega-hydroxyceramide to membrane proteins. Therefore plays a crucial role in the synthesis of corneocytes lipid envelope and the establishment of the skin barrier to water loss. In parallel, it may have a signaling function in barrier formation through the production of hepoxilins metabolites. Plays also a role in adipocyte differentiation through hepoxilin A3 and hepoxilin B3 production which in turn activate PPARG. Through the production of hepoxilins in the spinal cord, it may regulate inflammatory tactile allodynia.