Rabbit anti-Human Acid Phosphatase/ACP1 Polyclonal Antibody | anti-ACP1 antibody

Anti-Acid Phosphatase/ACP1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8104423

• Gene Names: ACP1; HAAP; LMWPTP; LMW-PTP
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: Acid Phosphatase/ACP1; Polyclonal Antibody; Anti-Acid Phosphatase/ACP1 Antibody; Rabbit Polyclonal; ACP1/LMW-PTP Antibody; Rabbit PAb; Antigen Affinity Purified; acid phosphatase 1; soluble; Anti-HAAP Antibody; anti-ACP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human ACP1/LMW-PTP
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-ACP1 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; IHC-P: 1:1000-1:4000; ICC/IF: 1:300-1:10,000; IP: 1-4uL/mg of lysate
• Immunogen: Recombinant Human ACP1/LMW-PTP Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human ACP1/LMW-PTP (rh ACP1/LMW-PTP). ACP1/LMW-PTP specific IgG was purified by Human ACP1/LMW-PTP affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-ACP1 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:18 kDaObserved band size:18 kDa)

Immunohistochemistry (IHC)

(Immunochemical staining of human ACP1 in human kidney with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).)

Immunofluorescence (IF)

(Immunofluorescence staining of ACP1 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti- ACP1 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.)

Immunoprecipitation (IP)

(ACP1 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg HepG2 Whole Cell LysateLane C:0.5 mg Hela Whole Cell Lysate4 uL anti-ACP1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-ACP1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 18 kDaObserved band size: 18 kDa)

Related Product Information for anti-ACP1 antibody

The low molecular weight phosphotyrosine phosphatase (LMW-PTP), also known as Acid phosphatase 1 (ACP1), belongs to the low molecular weight phosphotyrosine protein phosphatase family are involved in the regulation of important physiological functions, including stress resistance and synthesis of the polysaccharide capsule. ACP1/LMW-PTP is an enzyme involved in platelet-derived growth factor-induced mitogenesis and cytoskeleton rearrangement. LMW-PTP is able to specifically bind and dephosphorylate activated PDGF receptor, thus modulating PDGF-induced mitogenesis. In vitro, LMW-PTP was found to efficiently dephosphorylate activated FcgammaRIIA and LAT, but not Syk or phospholipase Cgamma2. The overexpression of LMW-PTP inhibited activation of Syk downstream of FcgammaRIIA and reduced intracellular Ca(2+) mobilization. It been demonstrated that LMW-PTP is responsible for FcgammaRIIA dephosphorylation, and is implicated in the down-regulation of cell activation mediated by this ITAM-bearing immunoreceptor. In addition, ACP1 is a highly polymorphic phosphatase that is especially abundant in the central nervous system and is known to be involved in several signal transduction pathways.

Product Categories/Family for anti-ACP1 antibody

Phosphatases and Regulators

References

Cirri P, et al. (1998) Low molecular weight protein-tyrosine phosphatase tyrosine phosphorylation by c-Src during platelet-derived growth factor-induced mitogenesis correlates with its subcellular targeting. J Biol Chem. 273(49): 32522-7.Chiarugi P, et al. (2002) Insight into the role of low molecular weight phosphotyrosine phosphatase (LMW-PTP) on platelet-derived growth factor receptor (PDGF-r) signaling. LMW-PTP controls PDGF-r kinase activity through TYR-857 dephosphorylation. J Biol Chem. 277(40): 37331-8.Bottini N, et al. (2002) Convulsive disorder and the genetics of signal transduction; a study of a low molecular weight protein tyrosine phosphatase in a pediatric sample. Neurosci Lett. 333(3): 159-62.Musumeci L, et al. (2005) Low-molecular-weight protein tyrosine phosphatases of Bacillus subtilis. J Bacteriol. 187(14): 4945-56.Mancini F, et al. (2007) The low-molecular-weight phosphotyrosine phosphatase is a negative regulator of FcgammaRIIA-mediated cell activation. Blood. 110(6): 1871-8.

NCBI and Uniprot Product Information

• NCBI GI #: 77415343
• NCBI GeneID: 52
• Molecular Weight: 12,230 Da
• NCBI Official Full Name: Acid phosphatase 1, soluble
• NCBI Official Synonym Full Names: acid phosphatase 1, soluble
• NCBI Official Symbol: ACP1
• NCBI Official Synonym Symbols: HAAP; LMWPTP; LMW-PTP
• NCBI Protein Information: low molecular weight phosphotyrosine protein phosphatase
• Protein Family: Adult cuticle protein

NCBI Description

The product of this gene belongs to the phosphotyrosine protein phosphatase family of proteins. It functions as an acid phosphatase and a protein tyrosine phosphatase by hydrolyzing protein tyrosine phosphate to protein tyrosine and orthophosphate. This enzyme also hydrolyzes orthophosphoric monoesters to alcohol and orthophosphate. This gene is genetically polymorphic, and three common alleles segregating at the corresponding locus give rise to six phenotypes. Each allele appears to encode at least two electrophoretically different isozymes, Bf and Bs, which are produced in allele-specific ratios. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Aug 2008]