Rabbit anti-Mouse, Human AcCoA Carboxylase Polyclonal Antibody | anti-ACC antibody

AcCoA Carboxylase (Ser79), Phosphorylated (ACC, Acetyl Coenzyme A Carboxylase) (MaxLight 550)

Cat. Num. AAA6460977

• Gene Names: ACACA; ACC; ACAC; ACC1; ACCA; ACACAD
• Reactivity: Mouse, Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: AcCoA Carboxylase; Polyclonal Antibody; AcCoA Carboxylase (Ser79); Phosphorylated (ACC; Acetyl Coenzyme A Carboxylase) (MaxLight 550); anti-ACC antibody

• Host: Rabbit
• Reactivity: Mouse, Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes AcCoA Carboxylase phosphorylated at Ser79, Mr 257kD. Species Crossreactivity: human.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.
• Sequence Length: 2383

• Applicable Applications for anti-ACC antibody: Western Blot (WB)
• Application Notes: WB: 1:500 detects phosphorylated AcCoA Carboxylase in lysate from lambda phosphatase-treated and untretated A375 cells.; Applications are based on unconjugated antibody.
• Immunogen: Synthetic linear peptide corresponding to aa73-85, C-HMRSSM[pS]GLHLVK, from rat AcCoA carboxylase conjugated to KLH (NP_942131). Species sequence homology: bovine, canine, chicken, rat and mouse
• Conjugate: MaxLight550
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap

Western Blot (WB)

(Western blot analysis of extracts of various cells, using CYCS antibody.)

Western Blot (WB)

(Western blot analysis of extracts of various cells, using CYCS antibody.)

Western Blot (WB)

(Western blot analysis of extracts of various cells, using CYCS antibody.)

Western Blot (WB)

(Western blot analysis of extracts of various cells, using CYCS antibody.)

Testing Data

()

Western Blot (WB)

(Western blot analysis of extracts of various cells, using CYCS antibody.)

Western Blot (WB)

(Western blot analysis of extracts of various cells, using CYCS antibody.)

Product Categories/Family for anti-ACC antibody

Antibodies; Coenzyme A

References

1. Kemp, B.E., et al., Trends Biochem. Sci. 24: 22-25 (1999). 2. Chen, Z.P., et al., Am. J. Physiol. Endocrinol. Metab. 279: E1202-E1206 (2000). 3. Andersson, U., et al., J. Biol. Chem. 279: 12, 005-12, 008 (2004). 4. Chan, A.Y., et al., J. Biol. Chem. 279: 32, 771-32, 779 (2004). 5. Miyazaki, M., et al., J. Biol. Chem. 279: 35, 017-35, 024 (2004). 6. Orci, L., et al., al. (2004). Proc Natl Acad Sci U.S.A. 101: 2058-63. 7. Nagata, D., et al., al. (2003). J Biol Chem. 278: 31000-6.

NCBI and Uniprot Product Information

• NCBI GI #: 38679960
• NCBI GeneID: 31
• NCBI Accession #: NP_942131
• NCBI GenBank Nucleotide #: NP_942131.1
• UniProt Accession #: Q13085
• NCBI Official Full Name: acetyl-CoA carboxylase 1 isoform 1
• NCBI Official Synonym Full Names: acetyl-CoA carboxylase alpha
• NCBI Official Symbol: ACACA
• NCBI Official Synonym Symbols: ACC; ACAC; ACC1; ACCA; ACACAD
• NCBI Protein Information: acetyl-CoA carboxylase 1
• UniProt Protein Name: Acetyl-CoA carboxylase 1
• UniProt Gene Name: ACACA
• UniProt Synonym Gene Names: ACAC; ACC1; ACCA; ACC1
• UniProt Entry Name: ACACA_HUMAN

NCBI Description

Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ACC1: a subunit of acetyl-CoA carboxylase (ACC), a multifunctional enzyme system. Catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. Phosphorylation by AMPK or PKA inhibits the enzymatic activity of ACC. ACC-alpha is the predominant isoform in liver, adipocyte and mammary gland. ACC-beta is the major isoform in skeletal muscle and heart. Phosphorylation regulates its activity.

Protein type: Ligase; EC 6.3.4.14; EC 6.4.1.2; Carbohydrate Metabolism - propanoate; Carbohydrate Metabolism - pyruvate; Lipid Metabolism - fatty acid biosynthesis

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: mitochondrion; cytoplasm; nucleolus; cytosol; actin cytoskeleton

Molecular Function: protein binding; acetyl-CoA carboxylase activity; metal ion binding; biotin carboxylase activity; ATP binding

Biological Process: vitamin metabolic process; acetyl-CoA metabolic process; multicellular organismal protein metabolic process; tissue homeostasis; positive regulation of cellular metabolic process; energy reserve metabolic process; lipid homeostasis; triacylglycerol biosynthetic process; carnitine shuttle; cellular lipid metabolic process; water-soluble vitamin metabolic process; fatty acid biosynthetic process; biotin metabolic process; protein homotetramerization

Disease: Acetyl-coa Carboxylase Deficiency