Immunohistochemistry (IHC)
(At 1/100 staining mouse heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
Rabbit ACC1 Polyclonal Antibody | anti-ACC1 antibody
Phospho-ACC1 (Thr1196) Antibody
Predicted: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
Predicted: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
IHC: 1:50-1:200
ELISA (Peptide): 1:20000-1:40000
Immunohistochemistry (IHC)
(At 1/100 staining mouse heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
Immunohistochemistry (IHC)
(At 1/100 staining mouse heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
Immunohistochemmistry (IHC)
(At 1/100 staining human gastric cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
Immunohistochemmistry (IHC)
(At 1/100 staining human gastric cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
Western Blot (WB)
(Western blot analysis of Phospho-ACC1 (Thr1196) in lysates of 293, using Phospho-ACC1 (Thr1196) Antibody.)
Western Blot (WB)
(Western blot analysis of Phospho-ACC1 (Thr1196) in lysates of 293, using Phospho-ACC1 (Thr1196) Antibody.)
Post Translational Modifications: Phosphorylation on Ser-1263 is required for interaction with BRCA1.
Subunit Structure: Monomer, homodimer, and homotetramer. Can form filamentous polymers. Interacts in its inactive phosphorylated form with the BRCT domains of BRCA1 which prevents ACACA dephosphorylation and inhibits lipid synthesis. Interacts with MID1IP1; interaction with MID1IP1 promotes oligomerization and increases its activity.
NCBI and Uniprot Product Information
Predicted: 266 kDa
NCBI Description
Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
ACC1: a subunit of acetyl-CoA carboxylase (ACC), a multifunctional enzyme system. Catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. Phosphorylation by AMPK or PKA inhibits the enzymatic activity of ACC. ACC-alpha is the predominant isoform in liver, adipocyte and mammary gland. ACC-beta is the major isoform in skeletal muscle and heart. Phosphorylation regulates its activity.
Protein type: Ligase; EC 6.3.4.14; EC 6.4.1.2; Carbohydrate Metabolism - propanoate; Carbohydrate Metabolism - pyruvate; Lipid Metabolism - fatty acid biosynthesis
Chromosomal Location of Human Ortholog: 17q21
Cellular Component: mitochondrion; cytoplasm; nucleolus; cytosol; actin cytoskeleton
Molecular Function: protein binding; acetyl-CoA carboxylase activity; metal ion binding; biotin carboxylase activity; ATP binding
Biological Process: vitamin metabolic process; acetyl-CoA metabolic process; multicellular organismal protein metabolic process; tissue homeostasis; positive regulation of cellular metabolic process; energy reserve metabolic process; lipid homeostasis; triacylglycerol biosynthetic process; carnitine shuttle; cellular lipid metabolic process; water-soluble vitamin metabolic process; fatty acid biosynthetic process; biotin metabolic process; protein homotetramerization
Disease: Acetyl-coa Carboxylase Deficiency
Research Articles on ACC1
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Product Notes
The ACC1 acaca (Catalog #AAA9610726) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-ACC1 (Thr1196) Antibody reacts with Human, Mouse, Rat Predicted: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's ACC1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200 ELISA (Peptide): 1:20000-1:40000. Researchers should empirically determine the suitability of the ACC1 acaca for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ACC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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