Rabbit anti-Human ACAT1 Polyclonal Antibody | anti-ACAT1 antibody

ACAT1, ID (Acetyl-CoA Acetyltransferase, Mitochondrial, Acetoacetyl-CoA Thiolase, T2, ACAT, MAT)

Cat. Num. AAA6002453

• Gene Names: ACAT1; T2; MAT; ACAT; THIL
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: ACAT1; Polyclonal Antibody; ID (Acetyl-CoA Acetyltransferase; Mitochondrial; Acetoacetyl-CoA Thiolase; T2; ACAT; MAT); Anti -ACAT1; anti-ACAT1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ACAT1. Species sequence homology: mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS (without Mg2+, Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-ACAT1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: ELISA: 1:40,000; Western Blot: 1:500-1:1000; Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
• Immunogen: Synthetic peptide corresponding to human ACAT1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Liver, Hephatocytes: Formalin-Fixed, Paraffin-Embedded (FFPE))

Western Blot (WB)

(Western blot analysis of extracts from HepG2/Jurkat/293/A549 cells, using ACAT1 antibody. The lane on the right is treated with the synthesized peptide.)

Related Product Information for anti-ACAT1 antibody

The ACAT1 mRNA encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. The ACAT gene spans approximately 27kb and contains 12 exons interrupted by 11 introns. Defects in this gene are associated with the alpha-methylacetoaceticaciduria disorder, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.

Product Categories/Family for anti-ACAT1 antibody

Antibodies; Abs to Enzymes; Acetyltransferase

NCBI and Uniprot Product Information

• NCBI GI #: 4557237
• NCBI GeneID: 38
• NCBI Accession #: NP_000010.1
• NCBI GenBank Nucleotide #: NM_000019.3
• UniProt Accession #: P24752; B2R6H1
• Molecular Weight: 45,200 Da
• NCBI Official Full Name: acetyl-CoA acetyltransferase, mitochondrial
• NCBI Official Synonym Full Names: acetyl-CoA acetyltransferase 1
• NCBI Official Symbol: ACAT1
• NCBI Official Synonym Symbols: T2; MAT; ACAT; THIL
• NCBI Protein Information: acetyl-CoA acetyltransferase, mitochondrial; acetoacetyl-CoA thiolase; acetoacetyl Coenzyme A thiolase; acetyl-Coenzyme A acetyltransferase 1; mitochondrial acetoacetyl-CoA thiolase
• UniProt Protein Name: Acetyl-CoA acetyltransferase, mitochondrial
• UniProt Gene Name: ACAT1
• UniProt Synonym Gene Names: ACAT; MAT
• UniProt Entry Name: THIL_HUMAN

NCBI Description

This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq, Feb 2009]

Uniprot Description

ACAT1: Plays a major role in ketone body metabolism. Defects in ACAT1 are a cause of 3-ketothiolase deficiency (3KTD); also known as alpha- methylacetoaceticaciduria. 3KTD is an inborn error of isoleucine catabolism characterized by intermittent ketoacidotic attacks associated with unconsciousness. Some patients die during an attack or are mentally retarded. Urinary excretion of 2-methyl-3- hydroxybutyric acid, 2-methylacetoacetic acid, triglylglycine, butanone is increased. It seems likely that the severity of this disease correlates better with the environmental or acquired factors than with the ACAT1 genotype. Belongs to the thiolase family.

Protein type: Amino Acid Metabolism - lysine degradation; Mitochondrial; Secondary Metabolites Metabolism - terpenoid backbone biosynthesis; Acetyltransferase; Lipid Metabolism - fatty acid; Amino Acid Metabolism - tryptophan; Carbohydrate Metabolism - propanoate; Lipid Metabolism - synthesis and degradation of ketone bodies; EC 2.3.1.9; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Carbohydrate Metabolism - butanoate; Carbohydrate Metabolism - pyruvate

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial inner membrane

Molecular Function: protein homodimerization activity; enzyme binding; acetyl-CoA C-acetyltransferase activity; metal ion binding; coenzyme binding

Biological Process: response to starvation; response to hormone stimulus; ketone body catabolic process; ketone body biosynthetic process; brain development; branched chain family amino acid catabolic process; cellular lipid metabolic process; ketone body metabolic process; liver development; response to organic cyclic substance; protein homooligomerization

Disease: Alpha-methylacetoacetic Aciduria